棒状杆菌固定化细胞生产L(+)-酒石酸

Production of L(+)-tartaric Acid by Immobilized Corynebacterium sp.JZ-1

以卡拉胶为载体,固定化棒状杆菌(Corynebacteriumsp.)JZ1菌株细胞,再经活化处理,顺式环氧琥珀酸水解酶(ESH)酶活力总回收率在100%以上。摇瓶反应10批,酶活力没有明显降低。1500L酶柱中连续运行90d,酶稳定性很好。固定化后酶反应的最适温度(45℃)和最适pH(90)没有改变,而热稳定性。

Corynebacterium sp. JZ 1 cells,which showed a high enzymatic activity of cis epoxysuccinate hydrolase,were immobilizde by entrapping in κ carrageenan,then treated with some active agents.The recovery of enzyme was over 100%.In shake flask,the stability of immobilized cells was satisfactory after they reacted with disodium cis epoxysuccinate for 10 batches.The same result was attained in column(1500L) with the substrate fed in continuously for 90 days.The optimum pH value of the immobilized cells was 9.0,the optimum temperature was 45℃.