白杄体细胞胚悬浮培养的动力学研究

Studies on Kinetics of Somatic Embryo Suspension Culture in Picea meyeri Rehd.et Wils.

白(ＰｉｃｅａｍｅｙｅｒｉＲｅｈｄ.ｅｔＷｉｌｓ.)是我国特有的云杉属树种,在林业生产和环境绿化中均具有重要地位。其体细胞胚胎发生的研究,一方面可用于优良种质的大规模快速繁殖,为植树造林和园林绿化提供优质苗木;另一方面可作为遗传转化的再生系统,进行树种遗传...

Embryogenic calli were produced from mature zygotic embryos of Picea meyeri Rehd.et Wils.which were cultured on modified LP medium containing 30 g/L sucrose,2 mg/L 2,4 D and 1 mg/L 6 BA.The growth rate of embryogenic calli could be improved by way of suspension culture in medium MS containing 20 g/L sucrose,1 mg/L 2,4 D and 1 mg/L KT.The optimum values of initial cell density and shake rate of flask in suspension culture were 3.0%(fresh weight)and 150 r/min respectively.Several parameters which might be correlated with growth were determined over a 10 day period.Maximum growth in terms of fresh weight,dry weight and number of ESM occurred during 6～10 day.pH and conductivity attained the lowest point during 6～8 day.A lot of somatic embryos were obtained following transfer of the suspension cultures to medium MS containing 5 mg/L ABA.Mature somatic embryos grew into plantlets with well developed cotyledons after being cultured on 1/2 MS medium supplemented with 0.5% activated charcoal but without hormone.