摘要
应用玻璃针将脂质体包裹的质粒pEGFP-C1通过睾丸输出管注射到4周龄昆明(KM)小白鼠睾丸曲精细管内。共注射6只小鼠,其中一只注射后马上做石蜡切片观察转染液注射入曲细精管内的情况,其他5只继续饲养,1周后取1只小鼠培养睾丸的支持细胞,观察是否有荧光出现。4周后,用PCR法检测剩余4只小鼠睾丸组织曲细精管基因组中的外源DNA,4只都显示为阳性。表明用睾丸输出小管注射法将外源基因导入小鼠睾丸是一条简便可行的新途径。
Using glass needle plasmid pEGFP-C1 liposome-encapsulated was injected into the testicular efferent of 4 weeks male KM mice. Do immediately after an injection of paraffin sections to obserne the transfeetion solution was injected into the seminiferous tules, remanent mouse to continue to raise, after a week, take one mouse cultivating testicular sertoli cells. After four weeks, exogenous DNA in the PCR method for detecting the remaining four mouse testis seminiferous tubules genome, all as positive. The result indicated that the data in this study to transgenie animals could be established by testicular efferent injection.
出处
《天津农业科学》
CAS
2013年第4期34-37,共4页
Tianjin Agricultural Sciences
基金
国家转基因重大专项(2012ZX08008-003)
