PD-1表达与慢性乙型肝炎病毒感染病理的相关性分析

Analysis on correlationship between PD-1 expression and pathology of chronic hepatitis B virus infection

目的研究程序性死亡因子-1(programmed death1,PD-1)与慢性乙型病毒性肝炎病理的相关性,探讨PD-1在慢性HBV感染病情进展中的作用。方法对84例慢性乙型病毒性肝炎患者行肝组织活检,肝组织标本经10%福尔马林液固定、石蜡包埋切片后,以HE染色评判肝组织炎症活动度(G)和肝纤维化程度(S)。采用免疫组织化学染色SP法检测肝组织中PD-1的表达,以红色为阳性表达区域,每张切片在高倍镜下(400×)选取5个不连续视野进行图像采集,采用Image-Pro Plus6.0图像分析系统进行测量分析,以积分光密度(integral optical density,IOD)值表示PD-1在肝组织中的表达水平。结果 PD-1表达强度与肝组织炎症活动度及纤维化程度均呈正相关,相关系数分别为0.898(P<0.001)、0.700(P<0.001)。肝组织炎症活动度G0～G4级组间,PD-1的表达差异均有统计学意义(P<0.05)。肝组织纤维化S2期与S3期、S3期与S4期间PD-1的表达,差异无统计学意义(P>0.05),其余各纤维化分期之间PD-1的表达,差异均有统计学意义(P<0.05)。结论肝组织内的PD-1表达水平与CHB患者肝脏炎症及纤维化发生、进展呈正相关。测定PD-1的IOD值有助于辅助评判肝组织炎症活动度及肝纤维化程度,可以作为评判肝炎病理情况的参考指标。

Objective To study the death 1 （PD-1） and the pathology chronic HBV infection. Methods correlationship between the expression of programmed of chronic hepatitis B, and explore the role of PD-1 in Liver biopsies were performed in all 84 patients with chronic hepatitis B, the liver tissues were fixed by 10% formalin and paraffin-embedded sections were stained by HE, and the hepatic inflammatory activity grade and fibrosis stages were evaluated. Immunohistochemical SP was applied to detect the expression of PD^I in liver tissues. The positive expression means that red staining was found in five discontinuous visual fields under high magnification （400 ~ ）. The results were analyzed by Image Pro Plus 6.0 software. The expression intensity of PD-1 in liver tissues were measured by integral optical density （IOD） titres. Results The expression of PD-1 was positively correlated with hepatic inflammatory activity grade and fibrosis stage, with correlation coefficient as 0.898 （P 〈 0.001） and 0.700 （P 〈 0.01） respectively. There were significant differences of PD-1 expression among the hepatic inflammatory activity grades of each groups （P 〈 0.05）. The expression of PD-1 were significantly different among the fibrosis stages of each group （P 〈 0.05）, except S2 and S3, S3 and S4 （P 〉 0.05）, Conclusions PD-I expression in liver tissues are positively correlated with the hepatic inflammatory activity grade and fibrosis stage. Detection of PD-1 is worthy of evaluating hepatic inflammatory activity and fibrosis stages, meanwhile, it can provide valuable reference for assessment in hepatitic pathology.