小鼠卵母细胞wee 1和cdc25 mRNART-PCR方法的建立

The Establishment of the RT PCR Using for wee1 and cdc 25 mRNA of Mouse Oocytes

在没有现成的哺乳动物卵母细胞 wee l、cdc 2 5m RNA的 RT-PCR方法可借鉴的情况下 ,经过反复摸索 ,终于选出了 1对适宜的 wee l c DNA引物和 1对 cdc2 5c DNA引物 ,并对 RT-PCR条件进行了优化试验 ,成功地建立起了一套稳定的 wee l、cdc2 5m RNA的 RT-PCR方法 ,为研究哺乳动物卵母细胞 。

We chose a pair of suitable wee 1 cDNA primer from 56 pairs of primers and a pair of suitable cdc 25 cDNA primer from 30 pairs of primers designed by computer (Goldkey programma) and improved RT PCR reactive conditions; thus we established the RT PCR using for wee 1 and cdc 25 mRNA of mouse oocytes. This RT PCR laid solid foundation for researching expression of mammalian oocyte genes, also mammalian early embryonic genes.