绿花椰菜片剂中莱菔硫烷含量测定及对急性酒精性肝损伤的保护作用

Quantitative determination of sulforaphane from broccoli extract tablets and its role in the protection of acute liver injury induced by ethanol

采用高效液相色谱法测定绿花椰菜水提物中莱菔硫烷的含量;以C57BL/6小鼠建立急性酒精性肝损伤模型,用苏木精-伊红染色法(hematoxylin-eosin staining,HE)检测莱菔硫烷对小鼠肝脏病理学的影响;采用半自动生化分析仪测定莱菔硫烷对肝脏丙氨酸氨基转移酶(alanine aminotransferase,ALT)、天门冬氨酸氨基转移酶(aspartate aminotransferase,AST)和碱性磷酸酶(alkaline phosphatase,ALP)活性的影响,以探讨绿花椰菜中的活性成分莱菔硫烷对急性酒精性肝损伤的保护作用.结果表明:绿花椰菜水提物中莱菔硫烷的含量为1.26mg/片;莱菔硫烷各剂量组均可明显降低酒精所致的肝质量增加,肝/体比值较酒精模型组明显下降(P<0.01);莱菔硫烷各剂量组均能减轻酒精对肝脏的病理学损伤,其中,高剂量莱菔硫烷组(80mg/kg)的作用尤为明显;莱菔硫烷能明显抑制酒精对肝脏ALT、AST和ALP等特征性酶的诱导作用,其中40和80mg/kg中、高剂量莱菔硫烷的保护作用更明显.说明莱菔硫烷能保护酒精对肝脏的损伤.

Sulforaphane （SFN） is an isothiocyanate （ITC） which exists as a precursor of glucosinolate （GS） in various cruciferous vegetables especially in broccoli. Sulforaphane has been regarded as a potential of anti cancer agent derived from diet, mostly because of its powerful induction of phase Ⅱ enzymes, and it is also acted as an antagonist of injury factors including lipopolysaccharide （LPS） and homocysteine （HCY）. The present study aims to explore whether broccoli extract, rich in sulforaphane, can protect the acute liver injury induced by ethanol in mice or not. The quantity of sulforaphane in broccoli extract was detected by high performance liquid chromatography （HPLC）. Hematoxylin-eosin （HE） staining was used to determine the pathological changes in C57BL/6 mice model with the acute liver injury induced by ethanol. The activities of alanine aminotransferase （ALT）, aspartate aminotransferase （AST） and alkaline phosphatase （ALP） in serum of mice were measured using semi-automatic biochemical analyzer. The results showed that the quantity of sulforaphane in the broccoli extract was 1.26 mg/tablet. Liver masses in all sulforaphane treatment groups were obviously reduced which were presented as the sharply decreased ratio of liver mass/body mass. The seriously pathological evidence was observed in ethanol treatment group, while less changes were seen in the sulforaphane treatment groups. Sulforaphane could dramatically inhibit the activities of ALT, AST and ALP in serum of mice induced by ethanol, with more obvious suppression in the moderate （40 mg/kg） and high-dose （80 mg/kg） groups, respectively. The result above indicates that the sulforaphane from broccoli extract tablets is able to protect the liver injury induced by ethanol.