摘要
目的建立测定注射用硫普罗宁中硫普罗宁含量的高效液相色谱法。方法采用waters C18色谱柱(150 mm×4.6 mm,5μm),水(磷酸调节pH为2.2)-乙腈(90∶10)为流动相,流速为1.0 mL/min,检测波长为210 nm。结果硫普罗宁质量浓度在25.01～400.16μg/mL的范围内与峰面积线性关系良好(r=0.999 9),平均回收率为100.01%,RSD为0.55%(n=9)。结论该方法简便、快速、准确,可用于测定注射用硫普罗宁中硫普罗宁的含量。
Objective To establish a HPLC method for the determination of tiopronin in Tiopronin Injection. Methods The analysis was performed on the Waters C18 column（150 mm×4.6 mm,5 μm）,using the mobile phase of water （adjusting pH to 2.2 with phosphoric acid） - acetonitrile （90 ： 10）. The flow rate was 1.0 mL/min. The detection wavelength was 210 nm. Results The linear range of tio- pronin was 25.01-400. 16 μg/mL（r=0. 999 9）.The average recovery rate was 100.01%, RSD 0. 55% （n=9）. Conclusion This method is simple,quick,accurate and can be used to determine tiopronin in Tiopronin Injection.
出处
《中国药业》
CAS
2013年第6期64-65,共2页
China Pharmaceuticals
