糖尿病大鼠体外循环术后认知功能变化及海马NF-κBp65蛋白的表达

Influence of α-lipoicacid to Cognition Function Impairment and NF-κBp65 Expression of Hippocampus after Cardiopulmonary Bypass in Diabetic rats

目的探讨糖尿病大鼠体外循环(cardiopulmonary bypass,CPB)术后认知功能的变化。方法将SD大鼠随机分成非糖尿病CPB组(A组)及糖尿病CPB组(B组)。腹腔一次性注射1%STZ(溶于pH 4.2,0.1%mol/L柠檬酸-柠檬酸钠缓冲液)60mg/kg建立糖尿病模型,糖尿病模型建立后第7周建立CPB模型。观察糖尿病大鼠CPB后认知功能的变化以及海马NF-κBp65蛋白的表达,抽取静脉血用ELISA法检测TNF-α及IL-10含量。结果各组大鼠术前认知功能测试无显著性差异(P>0.05),体外循环后5天各组差异显著,糖尿病CPB组(B组)电击次数明显增加(P<0.05,P<0.01),差别有显著性。B组大鼠脑组织海马NF-κBp65蛋白阳性细胞数明显增多,差异有统计学意义(P<0.01)。两组大鼠血清TNF-α及IL-10含量均有增高,B组大鼠增高更加明显,差异有统计学意义(P<0.05)。结论糖尿病可以引发并加重体外循环后大鼠脑内NF-κBp65蛋白过度表达,促使细胞因子释放,对脑组织产生损伤并出现认知功能障碍。糖尿病通过NF-κBp65蛋白途径参与糖尿病体外循环术后认知功能障碍的发生发展。

Objective Diabetic rats cardiopulmonary bypass （cardiopulmonary bypass, CPB） changes in postoperative cognitive function.. Methods SD rats were randomly divided into non-diabetic CPB group （A） and diabetes CPB group （B）. Intraperitoneal single injection of 1% STZ （dissolved in pH 4.2, 0.1% mol/L citric acid - sodium citrate buffer） 60mg/kg create a model of diabetes, diabetic model after 7 weeks in CPB model. Of diabetic rats after CPB cognitive function changes, and the hippocampus the NF-κBp65 protein expression to extract the venous blood ELISA was used to detect TNF-α and IL-10 levels. Results Tests of cognitive function in rats preoperative significant difference （P〉0.05）, five days after cardiopulmonary bypass groups significantly different, diabetes CPB group （B） the number of shocks increased significantly （P〈0.05, P〈0.01）, the difference is significant. Group B rat brain hippocampus the NF-κBp65 protein-positive cells increased significantly, and the difference was statistically significant （P〈0.01）. The two groups of serum TNF-α and IL-10 levels have increased more significantly increased B rats, the difference was statistically significant （P〈0.05）. Conclusions Diabetes can trigger and aggravate cardiopulmonary bypass the rat brain of NF-κBp65 protein overexpression, prompting the release of cytokines, brain tissue damage and cognitive dysfunction. Diabetes through NF-κBp65 protein pathways involved in the development of diabetes the extracorporeal circulation postoperative cognitive dysfunction.