雌激素对子宫内膜细胞EGF mRNA、EGFR mRNA表达的影响

Efforts of Estrogen on Expressions of EGF mRNA and EGFR mRNA in Endometrial Cells

目的探讨雌激素(E2)对体外培养人正常及异位子宫内膜细胞增殖能力、表皮生长因子(EGF)及其受体(EGFR)mRNA表达的影响。方法将体外培养的异位内膜细胞和正常内膜细胞分为E2短效组,在培养液中加入E2 50nmol/L30min;E2长效组,加入E2 50nmol/L5d;对照组,单纯加入培养液。用四甲基偶氮唑盐(MTT)比色法检测各组细胞的增殖能力,同时应用荧光定量PCR技术检测各组细胞中EGF mRNA及EGFRmRNA的表达水平。结果经雌激素刺激后,异位及正常内膜细胞的增殖能力增加,细胞中EGF mRNA、EGFR mRNA表达较对照组降低(P<0.05),但2种细胞的E2短效和长效组间的差异均无统计学意义(P>0.05)。结论雌激素可促进分泌期正常和异位内膜细胞的增生及分化,且雌激素作用时间长短并无明显不同。

Objective To clarify the effects of estrogen （E2） on the proliferation ability of in vitro cultured human normal and ectopic endometrial cells and the expressions of epidermal growth factor （EGF） and its receptor （EGFR） in these cells. Methods The in vitro cultured human endometriosis cells and eutopic endometrium cells were both classified into E2 short-term group （with culture fluid stimulated by E2 50 nmol/L for 30 minutes）, E2 long-term group （with culture fluid stimulated by E2 50 nmol/L for 5 days） and control group （with culture fluid only）. The MTY method was applied to detect the proliferation ability of endometrial cells, while the expressions of EGF mRNA and EGFR mRNA in these cells was analyzed by PCR techniques. Results With long-term and short-term stimulation of estrogen, the proliferation ability of eutopic endometrium cells and endometriosis cells and the expression of EGF mRNA and EGFR mRNA were all enhanced compared with those of control （P 〈 0.05）. There were no significant differences in all the three parameters between E2 short-term and E2 long-term groups （P 〉 0.05）. Conclusion Estrogen can promote the proliferation and differentiation of ectopic endometrial cells and eutopic endometrial cells during their secretary phase. And there was no difference between long-term and short-term stimulation of estrogen.