脂氧素A4对大鼠子前期治疗作用的研究

Study of Therapeutic Effects of Lipoxin A4 on Preeclampsia in Rats

目的研究脂氧素A4(LXA4)对大鼠子前期的治疗作用。方法将30只受孕大鼠随机分内毒素组、脂氧素组、生理盐水(NS)组3组,每组10只。在大鼠孕第14天用大鼠尾静脉缓慢注射低剂量脂多糖(LPS)建立大鼠子日前期模型;脂氧素组在内毒素建模后给予LXA4治疗;NS组给予注射生理盐水作为对照。3组分别于孕前、孕第8、18天测定大鼠体质量、收缩压、24h尿蛋白、血浆LXA4及肿瘤坏死因子(TNF)-α水平。结果大鼠孕前、孕第8天,3组大鼠体质量、收缩压、24h尿蛋白、血浆LXA4、TNF-α水平差异无统计学意义(P>0.05)。大鼠孕第18天即注药后第4天,3组大鼠体质量差异无统计学意义(P>0.05);内毒素组与脂氧素组和NS组比较,收缩压、24h尿蛋白及血浆TNF-α水平升高(P<0.05),血浆LXA4水平下降(P<0.05);脂氧素组与NS组比较,收缩压、24h尿蛋白、血浆TNF-α、LXA4水平差异无统计学意义(P>0.05)。结论低剂量内毒素复制大鼠子前期模型方法成熟,LXA4对子前期大鼠模型有明显的治疗作用。

Objective To study the therapeutic effects of lipoxin A4 （LXA4） on preeclampsia in rats. Methods Thirty pregnant SD rats were randomly divided into three groups （n=10 for each group）： treatment with lipoxin A4 group, model of lipopolysaccharide （LPS） group and brine group. The model of LPS was induced by the injection of low dose LPS slowly in the tail vein of rats on the pregnancy of the 14th day. LXA4 was given for the treatment in lipoxin A4 group. Brine group was injected saline as comparison. The body weight, systolic blood pressure, 24-hour urine protein, level of plasma LXA4 and tumor necrosis factor （TNF-oL） were detected before pregnancy, day-8 and day-18 of gestation in three groups. Results There were no significant differences in the weight, systolic blood pressure, 24-hour urine protein, level of plasma LXA4 and TNF-a before pregnancy, day-8 and day-18 of gestation between three groups（P 〉 0.05）. There was no significant difference in body weight between three groups on the pregnancy of the 18th day（P 〉 0.05）. However, there were significant higher levels in the systolic blood pressure, 24-hour urine protein, level of plasma LXA4 and TNF-a in treatment with lipox- in A4 group and model of LPS group than those of brine group （P 〈 0.05）. Compare with the brine group, the level of LXA4 was significantly decreased in treatment with LXA4 group and model of LPS group （P 〈 0.05）. There were no significant differences in the systolic blood pressure, 24-hour urine protein, levels of plasma LXA4 and TNF-a between treatment with LXA4 group and brine group （P 〉 0.05）. Conclusion The method is mature to establish a model of preeclampsia in pregnant rats by given low dose LPS. LXA4 has a significant role in the treatment of preeclampsia in rat model.