摘要
目的 观察大鼠去卵巢 30 d时骨量、骨结构及血脂的变化。方法 17只 SD雌性大鼠随机分为 2组 :假手术组、去卵巢组。实验 30 d,处死大鼠取胫骨及血清 ,用骨计量学方法观察骨量和骨结构变化 ,并检测血清碱性磷酸酶 (AKP)、钙 (Ca)、磷 (P)、总胆固醇 (CH)、甘油三脂 (TG)。结果 去卵巢组与假手术组比 ,骨小梁面积和数量减少 (P<0 .0 1) ,骨小梁间隙增加 (P<0 .0 5) ;血液检验显示 AKP增加 (P<0 .0 1) ,CH增加 (P<0 .0 5) ,TG降低 (P<0 .0 1)。结论 本文结果显示 30 d去卵巢大鼠已出现典型的骨质疏松表现 ,伴有血脂的紊乱。
Objective To study the changes of rats bone volume and serum lipid on ovariectomized rats.Methods 17 female SD inbred rats were divided into 2 groups:sham ovx group and ovx group,30 days experiment period,the proximal tibial metaphysis(PTM)were processed undecalcified for quantitative bone histomorphometry and serum were collected to measure alkaline phosphatase(AKP),calcium(Ca),phosphorus(P),cholesterol(CH)and triglyceride(TG).Results Trabecular area percent(Tb.Ar%)and Trabecular number(Tb.N)of ovx rats decreased ( P <0.01),trabecular separation(Tb.Sp)increased( P <0.05)compared with sham ovx group.Compared with sham ovx group,the AKP level of ovx group increased ( P <0 01),Ca P has no change,CH increased ( P <0.05),TG decreased( <0.01).Conclusion The data suggests that 30 days after ovariectomy,rats'bone volume decreased,while serum CH level increased and TG level decreased.
出处
《中国骨质疏松杂志》
CAS
CSCD
2000年第2期42-43,86,共3页
Chinese Journal of Osteoporosis
基金
广东省卫生厅"五个一科教兴医工程"基金
