人黑色素瘤细胞系基因突变的研究

The study on genomic mutation of human melanoma cell lines

目的 揭示金属蛋白酶类 (MMPs) ,层粘连蛋白受体 (LN R)与人黑色素瘤细胞侵袭转移的关系 ,并探讨MMPs ,LN R用以判断肿瘤细胞侵袭转移的可能性。方法 通过流式细胞术 (FCM )定量研究和蛋白酶活性分析 (zy mography) ,对具有不同潜在转移能力的人黑色素瘤细胞进行研究。 结果 早期WM 3 5不产生MMPs ;WM 13 4 1B仅产生MMP 2不产生MMP 9;进展期WM 983A和远处转移瘤株WM 45 1既产生MMP 2又产生MMP 9。瘤细胞表面67KDLN R的荧光阳性率和全部细胞的平均荧光强度大小顺序为WM 45 1>WM 983A >WM 13 4 1B >WM 3 5。结论 MMPs和LN R与人黑色素瘤细胞系的侵袭转移能力获得之间存在着密切关系 ,并可作为一种较特异的肿瘤侵袭转移标记物被应用于肿瘤研究与治疗中。

? Objective To observe the relation of metalloproteinases(MMPs)、laminin receptor (LN R)with invasion and metastasis of human melanoma cell lines and probe into their probability to determine tumor invasion and metastasis.Methods FCM and the zymography were used to study the human melanoma cell lines with different metastatic potential.Results The early WM35 did not produce MMPs;WM1341B only produced 72KDa (MMP 2),not 92KDa(MMP 9),both WM983A at progressing stage and WM451 with the potential to disseminat produced 72KDa and 92KDa.The fluorescent positive rate of 67KD LN R on the surface of the melonoma cell and the average fluorescent intensity were in an order of WM451>MW983A>WM1341B>WM35.Conclusions MMPs and LN R closely correlats with the invasive ability for human melanoma cell lines,thus can be a specific marker for tumor metastasis in research and treatment of tumor. 〔