摘要
目的 通过观察刺梨汁对人白血病K5 62细胞生长的影响 ,探讨刺梨汁的抗癌作用。方法 以细胞计数和MTT法测定刺梨汁对细胞增殖的抑制作用 ,以测定细胞的乳酸脱氢酶释放量作为刺梨汁对细胞的毒性作用 ,并以K 5 62细胞形态的改变作为观察指标。结果 每mL培养液含 1μL刺梨汁即对肿瘤细胞增殖有明显的抑制作用 ,每mL培养液含 2 μL刺梨汁抑制作用达到最大 ,为 83 .4% ,再加大剂量抑制作用无明显升高 ,各种剂量的刺梨汁对细胞均无毒性 ,刺梨汁的加入使得细胞体积明显变小 ,细胞核形态发生改变 ,异染色质增多。结论 刺梨汁具有一定的抗癌作用。
? Objective To observe the effect of Rose roxburghii tratt juice (RRTJ) on K562 cells in vitrol.Methods Inhibitory effect of RRTJ on K562 cells was determined by counting alive cells and measuring MTT value.LDH release was measured to evaluate the virulence effect of RRTJ on the cells.The cytomorphological changes of K562 cells were observed in the electron microscope.Results RRTJ at the concentration of 1μL/mL expressed obviously inhibitory effect on the growth of K562 cells.The inhibitory effect at the concentration of 2μl/ml went up to its highest at 83.4%.RRTJ showed virulence to K562 cells.The karyon changed because of RRTJ attending.Conclusions RRTJ have anticancer function. 〔
出处
《中国肿瘤临床与康复》
2000年第4期32-34,共3页
Chinese Journal of Clinical Oncology and Rehabilitation
关键词
K562细胞
刺梨汁
抑制
K562 cell
rose roxburghii tratt juice(RRTJ)
inhibit