全身振动对去卵巢骨质疏松大鼠骨髓细胞p-GSK3β蛋白表达的调节作用

Regulating effects of whole-body vibration on protein expression of p-GSK3β in bone marrow cells of ovariectomized osteoporosis rats

本文旨在观察全身振动对去卵巢骨质疏松大鼠骨髓细胞Wnt/β-catenin信号通路的影响。将36只3月龄雌性Sprague Dawley(SD)大鼠,按体重随机分为假手术、去卵巢静止和去卵巢振动组。去卵巢10周后,去卵巢振动组大鼠每天接受2次振动(90Hz)刺激,每次15min。振动处理8周后,用双能X线骨密度仪在体检测大鼠体成分和骨密度,用Western blot检测骨髓细胞和骨髓基质细胞β-catenin和p-GSK3β蛋白的表达。结果显示,去卵巢振动组大鼠体脂重量和体脂含量显著低于去卵巢静止组,瘦体含量显著高于去卵巢静止组。去卵巢振动组大鼠胫骨近端骨密度显著高于去卵巢静止组,而全身、股骨远端和腰椎骨密度与去卵巢静止组相比无显著性差异。去卵巢振动组和去卵巢静止组的骨髓基质细胞β-catenin蛋白表达之间无显著性差异。去卵巢振动组骨髓细胞p-GSK3β蛋白的表达水平显著高于去卵巢静止组,而两组的骨髓基质细胞p-GSK3β蛋白表达之间无显著性差异。以上结果提示,全身振动能减缓去卵巢大鼠骨量的丢失,其机制涉及骨髓细胞p-GSK3β蛋白表达的上调。

The aim of this study was to investigate the effects of whole-body vibration on Wnt/β-catenin signaling pathway in bone marrow cells of ovariectomized osteoporosis rats. Thirty-six healthy 3-month old female Sprague Dawley （SD） rats were randomly divided into the following three groups by body weight： sham-operation （Sham）, ovariectomized （OVX）, and OVX whole-body vibra- tion （WBV） groups. Ten weeks after ovariectomization, the rats of WBV group received vibration treatment （90 Hz, 15 min） twice per day. At the end of 8-week vibration, the whole-body bone mineral density （BMD） and body composition were detected by dual energy X-ray absorptiometry （DEXA） in vivo. The protein expressions of β-catenin and p-GSK3β in both bone marrow cells and bone mar- row stromal ceils were detected by Western blot. The results showed that, compared with OVX group, WBV group showed decreased fat mass and fat mass content, as well as increased lean body mass content. The BMD of the proximal tibia in WBV group was signifi- cantly higher than that in OVX group, however, there was no difference of BMD in whole-body and other positions between the two groups. The β-catenin expression in bone marrow stromal cells showed no difference between OVX and WBV groups. The p-GSK3β expression of bone marrow cells was increased in WBV group compared with that in OVX group, whereas bone marrow stromal cells from two groups did not exhibit the difference of the p-GSK3β expression. These results suggest that whole body vibration can stimu- late the protein expression of p-GSK3β in bone marrow cells of ovariectomized osteoporosis rats, which could improve the bone loss induced by ovariectomization.