咖啡酸苯乙酯对雌性骨质疏松小鼠的实验研究

Experimental study of the effect of coffee acid ethyl benzene on female osteoporotic mice

目的采用骨生物力学、骨密度测定方法综合评价咖啡酸苯乙酯(Caffeic acid phenethyl esterCAPE)对去势小鼠骨质疏松影响的实验研究。方法取96只12周龄C57BL/6J雌性小鼠随机分16组,(每组6只)。分别研究CAPE预防骨质疏松部分:1周假手术组(sham PBS组)、去势组(ovx PBS组);4周、7周的sham PBS组、ovx PBS组、ovx CAPE(0.5 mg/kg)组、ovx CAPE(1 mg/kg)组。CAPE治疗骨质疏松部分:7周sham空白组和ovx空白组;造模7周后腹腔注射药物4周,包括11周sham PBS组、ovx PBS组、ovx CAPE(0.5 mg/kg)组、ovx CAPE(1 mg/kg)组。96只小鼠均用0.5%的戊巴比妥0.3 ml腹腔注射麻醉,5分钟后心脏采血,采用ELISA方法检测血清中雌激素水平。并对小鼠的双侧肱骨、脊柱进行取材,取双侧肱骨进行三点弯曲实验,采用美国HOLOGIC骨密度仪分析小鼠脊柱骨密度变化。结果 (1)血清中雌激素水平检测发现,所有ovx组雌激素较sham组显著降低(P<0.05)。(2)骨密度:预防部分4周ovx组的脊柱骨密度值较sham组、ovx0.5mg组及ovx1mg组明显降低(P<0.05)。(3)生物力学:预防部分1周、4周与治疗部分7周、11周ovx组较sham组、ovx0.5mg组、ovx1mg组的最大破坏应力指标显著降低(P<0.05)。结论咖啡酸苯乙酯能明显改善去势骨质疏松小鼠骨的力学性能,增加去势骨质疏松小鼠的骨密度,抑制骨吸收,从而达到预防和治疗骨质疏松的作用。

Objective To evaluate the effect of caffeic acid phenethyl ester （CAPE） on ovariectomized mice with osteoporosis comprehensively by measuring bone biomechanics and bone mineral density （BMD）. Methods A total of 96 12-week C57BL/6J mice were randomly divided into 16 groups （ n = 6 in each group）. Prevention of osteoporosis using CAPE： l-week sham group （sham PBS group）, ovariectomized group （OVX PBS group） ; 4-week and 7-week sham PBS group, OVX PBS group, OVX CAPE （0. 5 mg/ kg） group, and OVX CAPE （ 1 mg/kg） group. Treatment of osteoporosis using CAPE： 7-week sham blank group and OVX blank group, and after 4-week intraperitoneal injection, ll-week sham PBS group, OVX PBS group, OVX CAPE （0.5 mg/kg） group, and OVX CAPE （1 mg/kg） group. All the 96 mice were anesthetized with 0. 3 ml0. 5% pentobarbital intraperitoneal injection. Blood samples were taken from the heart 5 minutes later. Serum estrogen level was detected using ELISA method. Bilateral humerus and the spine were collected. Three-point bending experiment was performed on the bilateral humerus. BMD of the spine was tested using dual-energy X-ray absorptiometry （ HOLOGIC, USA）. Results Serum estrogen level in all OVX groups was significantly lower than that in sham group （P 〈 0. 05）. In prevention part, BMD in 4-week OVX group was significantly lower than that in sham group, OVX 0.5 mg group, and OVX l mg group （P 〈 0. 05）. On biomeehanies part, the maximum stress failure index in 1-week and 4-week OVXgroup in prevention part and 7-week and 11-week OVX group in treatment part was significantly lower than that in sham group, OVX 0. 5 nag group, and OVX lmg group （ P 〈 0.05 ）. Conclusion CAPE can obviously improve the mechanical properties of the bone in ovariectomized osteoporotic mice, increase the BMD of ovariectomized osteoporotic mice, and inhibit bone resorption, leading to the prevention and treatment of osteoporosis.