EB病毒抗体检测在不同年龄壮族鼻咽癌患者中的应用

Epstein-barr Virus Antibodies Response for Zhuang NPC Patients of Different Ages

目的探讨桂西地区壮族鼻咽癌患者EB病毒各年龄段Rta/IgG、VCA/IgA、VCA/IgG及Zta/IgG抗体的rA值和阳性率与年龄的关系。方法收集140例未经治疗的鼻咽癌患者和280例健康人的血清,用酶联免疫吸附法(ELISA)检测Rta/IgG、VCA/IgA、VCA/IgG及Zta/IgG抗体,分别计算各年龄段的抗体水平及阳性率并进行统计学分析。结果鼻咽癌患者各年龄段VCA/IgA抗体rA值和阳性率差异均有统计学意义(P<0.05)。在50岁以后年龄段的患者与健康人Rta/IgG、VCA/IgA抗体阳性率比较差异有统计学意义(P<0.05)。在50岁以后的年龄段患者与健康人Zta/IgG抗体阳性率比较差异无统计学意义(P>0.05)。结论壮族鼻咽癌患者与健康人EB病毒Rta/IgG、VCA/IgA及Zta/IgG抗体水平和阳性率比较存在年龄上的差异。在鼻咽癌的临床诊断和高危人群筛查中有必要根据不同年龄段的人群界定不同的阳性临界值。

Objective To evaluate the relationship between ages and the Rta/IgG,VCA/IgA,VCA/IgG and Zta/IgG values and positive rate of the Epstein-Barr virus（EBV） antibodies in Zhuang NPC patients at different ages in west Guangxi.Methods Serum samples obtained from140 patients with nasopharyngeal carcinoma（NPC） and from 280 healthy people were examined for the EBV antibodies including Rta/IgG,VCA/IgA,VCA/IgG and Zta/IgG by the enzyme-linked immnunosorbent assay（ELISA）.The positive rates and antibody levels of the NPC patients at different ages were analyzed statistically.Results Significant difference in VCA/IgA values and positive rates was found in the NPC patients at different ages（P0.05）.There was significant difference in the positive rates of Rta/IgG and VCA/IgA between the patients and healthy people above 50 years old（P0.05）.But no significant difference in the positive rates of Zta/IgG was found between them（P0.05）.Conclusion The serum levels and positive rates of EB virus antibodies（Rta/IgG,VCA/IgA and Zta/IgG） fluctuate with different ages.Different positive critical values of EBV antibodies for respective age groups should be set to facilitate NPC diagnosis and high-risk screening.