坎地沙坦酯对急性心肌梗死大鼠血管内皮功能的干预影响

Effects of candesartan cilexetil on vascular endothelial function in rats with acute myocardial infarction

目的:研究坎地沙坦酯(candesartan cilexetil,CAN)对急性心肌梗死(acute myocardial infarction,AMI)大鼠血管内皮功能的干预。方法:将大鼠随机分为AMI组、CAN组和假手术组,干预2周后处死,检测血压(blood pressure,BP)、血一氧化氮(nitric oxide,NO)、血管紧张素Ⅱ(angiotensionⅡ,AngⅡ)含量、一氧化氮合酶(nitric oxide synthase,NOS)活性、离体胸主动脉条片舒缩功能。结果:与假手术组比较,AMI组血清NO含量、NOS活性降低,胸主动脉内皮依赖性舒张(endothelium-dependent diastole,EDD)功能减退,血浆AngⅡ含量升高,差异有统计学意义(P=0.000 4)。经CAN干预后血清NO含量、NOS活性升高达假手术组水平,胸主动脉EDD显著改善(P=0.0009),同时血浆AngⅡ含量升高,各组BP并无明显变化。结论:CAN在不影响BP的前提下显著改善大鼠AMI后的血管内皮功能。

Objectives：To study the effects of candesartan cilexetil （CAN） on vascular endothelial function in rats with acute myocardial infarction. Methods：Rats were randomized into acute myocardial infarction （AMI） group, sham-operation group and CAN group. Rats were sacrificed at two weeks after the intervention and blood pressure（BP）,levels of blood nitric oxide（NO） and angiotension Ⅱ （Ang Ⅱ ）, activities of nitric oxide synthase（NOS）, endothelial vasomotor function of isolated thoracic aorta strips were explored. Re- suits：Compared with those in sham-operation group,levels of serum NO and activities of NOS were lower,function of endotheliumdependent diastoles（EDDs） was decreased seriously while levels of plasma Ang Ⅱ were distinctly higher in AMI group,with significant statistical differences（P=0.000 4）. After CAN treatment,levels of serum NO and activities of NOS were obviously higher,almost reaching the similar levels in sham-operation group;meanwhile function of EDDs in isolated aortic strips was improved greatly（P= 0.000 9） and levels of plasma Ang Ⅱ were increased significantly, however, BP in all groups was not affected. Conclusions ： CAN can greatly improve the disordered endothelial function of rats with acute myocardial infarction without affecting BP.