P_(53)、P_(21)ras、PCNA、c-erbB-2、nm23/NDPK在胃癌、大肠癌中表达的比较研究

COMPARATIVE STUDY ON THE EXPRESSION OF P 53 ,P 21 RAS, PCNA,C-ERBB-2 AND NM 23/NDPK IN GASTRIC AND CARCINOMAS OF LARGE INTESTINE

目的 :对胃肠癌P53 、P2 1ras、c -erbB - 2、nm2 3/NDPK ,PCNA过表达进行对比分析 ,探讨胃肠癌中多种基因单独、共同表达及肿瘤发生、发展的关系。方法 :采用免疫组织化学S -P法 ,同时检测 5 5例胃癌和 6 0例大肠癌中P53 、P2 1ras、c -erbB - 2、nm2 3/NDPK、PCNA的表达。结果 :P53 、P2 1ras、c -erbB - 2、PCNA过表达及nm2 3/NDPK低表达与胃肠癌浸润、转移有关 (P <0 0 5 ) ;两个及两个以上基因过表达在胃癌中为 72 7% ,大肠癌中70 %。胃肠癌中P53 与P2 1ras、c -erbB - 2、PCNA表达呈正相关 ,P2 1ras与c -erbB - 2、PCNA表达也呈正相关 ,nm2 3/NDPK与P53 、P2 1ras表达呈负相关。结论 :P53 、P2 1ras、PCNA、c -erbB - 2、nm2 3/NDPK可作为临床判断胃。

OBJECTIVE:Comparative analysis of P 53 ,P 21 ras,PCNA,C-erbB-2,nm23/NDPK expression in gastric and Carcinomas of large intestine to investigate the above genes alone and cooperative expression and the relation between them and development of gastric and carcinomas of large intestine. METHOD:The P 53 ,P 21 ras,PCNA,C-erbB-2 and nm23/NDPK expression in 55 cases of gastric carcinomas and 60 cases of Carcinomas of large intestine were detected by immunohistochemical S-P methed.RESULTS:The results showed that P 53 ,P 21 ras,PCNA and C-erbB-2 over-expression and nm23/NDPK low expression were correlated with infiltration and metastasis of gastric and colonic carcinomas.The rate of two and more over-expression was 72.7% (40 cases) of 55 gastric carcinomas and 70% (42 cases) of 60 carcinomas of large intestine (P<0 05). The P 53 expression was positively correlated with P 21 ras,PCNA adn C-erbB-2 expression and the P 21 ras expression was also positively correlated with PCNA and C-erbB-2 (P<0 05),but the nm23/NDPK expression was negatively Correlated with the P 53 and P 21 ras expression (P<0 05).CONCLUSION:P 53 ,P 21 ras,C-erbB-2,PCNA and nm23/NDPK can be helpful to judging biological behaviour of gastric and large intestine carcinomas.