游离脂肪酸通过刺激整合素连接激酶表达促进肾癌786-O细胞增殖

Free fatty acids induce proliferation of human renal carcinoma 786-O cell line via integrin-linked kinase

目的:探讨游离脂肪酸(free fatty acids,FFAs)异常对人肾癌786-O细胞增殖以及整合素连接激酶(inte-grin-linked kinase,ILK)蛋白表达的影响。方法:分别用0、0.05、0.1和0.2 mmol/L的油酸(oleic acid,OA)作用人肾癌786-O细胞48 h,其中0 mmol/L组作为对照组。利用MTT方法检测各组细胞增殖情况,流式细胞仪检测各组细胞凋亡情况,Western blot方法检测各组细胞中ILK、Akt和p-Akt蛋白的表达水平。结果:MTT检测结果显示,0.05、0.1和0.2 mmol/L OA组与对照组相比,细胞增殖活性显著增高(光密度0.657±0.056、0.682±0.028、0.718±0.042 vs.0.495±0.034,P均<0.001);而不同浓度的OA对786-O细胞的凋亡作用并不明显(凋亡率2.42%±0.25%vs.2.33%±0.87%vs.2.25%±0.51%,P=0.082);与对照组相比,OA组中的ILK、p-Akt蛋白表达显著上调。结论:FFAs通过刺激ILK/Akt通路可促进细胞增殖,可能与肾癌发生有关。

Objective：To investigate the effects of free fatty acids on cell proliferation and integrin-linked kinase （ILK） expression in human renal carcinoma 786-O cell line. Methods： The 786-O cells were exposed to normal medium and different concentrations of oleic acid （OA） carried by de-fatty bo-vine serum albumin （d-BSA）. The MTY assay and the flow cytometry assay were performed respectively for cell proliferation and apoptosis after the treatment with OA for 48 h. The expressions of ILK, Akt and p-Akt were detected by Western blot. Results： The MTT assay showed that the cell viabilities of O. 05 mmol/L, 0.1 mmoL/L and 0.2 mmol/L OA groups were increased gradually, as compared with the blank control （absorbance： 0.657 ±0. 056, 0. 682 ±0. 028, 0. 718 ±0. 042 vs. O. 495 ±0. 034; all P 〈 0. 001 ）. The effects of OA on cells apoptosis were not significant （apoptotic rates： 2.42% ± 0.25% vs. 2.33% ±.87% vs. 2.25% ±0.51%, P =0. 082）. After being treated with OA, the expressions of ILK and p-Akt were increased in 786-O cells. Conclusion： The results suggested that free fatty acids could promote the development of renal cell carcinoma via up-regulating ILK/Akt pathway, which may reveal the relations between metabolic disturbance and renal carcinoma to a certain extent.