UPLC法测定大豆制品及相关制剂中大豆异黄酮含量

Determination of Isoflavones in Soybean Products and Supplements by UPLC

目的:建立同时测定大豆制品及相关制剂中4种大豆异黄酮类化合物大豆苷、染料木苷、大豆苷元、染料木素含量的超高效液相色谱法。方法:色谱柱:Acquity UPLC BEH C18柱(50mm×2.1mm,1.7 m),流动相:A为体积分数0.2%甲酸溶液,B为乙腈,梯度洗脱;流速:0.4mL/min;检测波长:254nm,柱温:30℃。结果:线性范围:大豆苷0.625～40.0mg/L(r=1.000),染料木苷0.625～40.0mg/L(r=0.9999),大豆苷元0.04～2.56mg/L(r=0.9997),染料木素0.04～2.56mg/L(r=0.9996)。大豆和Natrol soy isoflavones中大豆苷、染料木苷、大豆苷元、染料木素平均回收率为98.5%～99.8%。结论:本方法简便、快速、灵敏、准确,适用于大豆制品及相关制剂中大豆异黄酮含量测定。

Objective： To develop an ultra-high performance liquid chromatography （UPLC） method for the determination of four major isoflavones such as daidzin, genistin, daidzein and genistein in soybean products and supplements. Methods： The analytes were separated on an Acquity UPLC BEH C18 column （50 mm×2.1 mm, 1.7μm） with a mobile phase consisting of 0.2% formic acid and acetonitrile in gradient mode, and UV detection at 254 nm. The flow rate was 0.4 mL/min. The column temperature was 30 ℃. Results： A good linear relationship within the range of 0.625 to 40.0 mg/L for daidzin （r = 1.000）, 0.625 to 40.0 mg/L for genistin （r = 0.9999）, 0.04 to 2.56 mg/L for daidzein （r = 0.9997） and 0.04 to 2.56 mg/L for genistein （r = 0.9996） was observed. The average recovery rates of daidzin, genistin, daidzein and genistein were 98.5%-99.8%. Conclusion： The developed method is fast, simple, sensitive, and accurate for the determination of four isoflavones in soybean products and supplements.