陕西省不同产地绿茶中6种活性成分含量的比较

Determination and Comparison of Six Active Compounds in Green Tea from Shaanxi Province

目的:采用高效液相色谱法HPLC,对陕西不同产区(安康、汉中和商洛)绿茶中茶氨酸(theanine)、没食子酸(gallic acid,GA)、咖啡碱(caffeine,CAF)、表没食子儿茶素(epicatechin gallate,EGC)、表儿茶素没食子酸(epicatechin gallate,ECG)及表没食子儿茶素没食子酸酯(epigallocatechin gallate,EGCG)的含量进行分析比较。方法:绿茶经热水提取后采用HPLC测定上述6种活性成分的含量,色谱柱(250mm×4.6mm,5μm),流动相为0.05%三氟乙酸溶液(A)、乙腈(B)梯度洗脱,流速为1mL/min,柱温25℃,检测波长:190nm和280nm。结果:3个产区中6种组分的含量在0.325～47.061mg/g之间,产区间各组分含量存在显著性差异(P<0.05),其中汉中绿茶中GA的平均含量最高,安康绿茶中茶氨酸、CAF、ECG、EGC、EGCG的平均含量最高。

Objective： To determine and compare the contents of theanine, gallic acid （GA）, CAF （CAF）, epicatechin gallate （EGC）, epigallocatechin gallate （EGCG） and epicatechin gallate （ECG） in tea samples from three regions of Shaanxi province by high performance liquid chromatography （HPLC）. Methods： Tea samples were extracted by hot water. The six active compounds were separated on a C18 column （250 mm × 4.6 mm, 5 μm） and examined by a photodiode array （PDA） detector at 190 nm and 280 nm with gradient elution at 25℃. The mobile phase was composed of trifluoroacetic acid （0.05%） and acetonilrile at the flow rate of 1 mL/min. Results： The contents of the six active compounds were in range of 0.325-47.061 mg/g. The level of GA was significantly higher （P 〈 0.05） in Hanzhong green tea, while the levels of other five active compounds were significantly higher （P 〈 0.05） in Ankang green tea.