结构型一氧化氮合酶基因表达在新生鼠缺氧性脑损伤中的作用

The Role of Intracellular cNOS mRNA Expression in Neonatal Rats with Hypoxic Brain Damage

目的 探讨结构型一氧化氮合酶 (cNOS)mRNA表达在新生鼠缺氧性脑损伤发病中的作用。方法 取新生SD大鼠 2 5只随机分组制作模型并行病理鉴定 ;采用RT -PCR测定脑缺氧 1h ,4h和对照组脑组织细胞内cNOSmRNA表达的变化 ,辉度扫描获取数值。结果 鼠缺氧 1h后脑组织中cNOSmRNA表达显著上升 (P <0 .0 1 ) ,4h组较 1h组下降 (P <0 .0 1 ) ,但仍显著高于对照组 (P <0 .0 1 )。结论 脑缺氧早期cNOSmRNA表达增加 ,由其合成的NO对受损脑组织可能起保护作用。

Objective To elucidate the role of cNOS mRNA expression in the pathogenesis of hypoxic brain injury after neonatal hypoxia. Methods RT PCR was used to measure cNOS mRNA gene expression in brain cells obtained from a neonatal rat model of hypoxia (n=25). Results The expression of cNOS mRNA in both 1 and 4 hour hypoxic groups was both increased compared with that in the control group (P<0.01), but it was decreased in the 4 hour group compared with the 1 hour group (P<0.01). Conclusions NO synthesized by cNOS mRNA in the early hypoxic phase might have some protective effect on hypoxic brain tissues.