三种宫内发育迟缓大鼠模型方法的比较

Evaluation of Three Rat Model of Intrauterine Growth Retardation

目的 对比常用的三种建立宫内发育迟缓 (IUGR)大鼠模型的方法 ,选择适用于儿科研究IUGR对生后生长发育和代谢远期影响的动物实验模型。方法 1 5只孕鼠平均分为 3组采用子宫动脉结扎法、更生霉素腹腔注射法、被动吸烟法建立IUGR动物模型 ,以自然分娩为结束妊娠的方式 ,比较仔鼠的体重 ,IUGR发生率及围产期死亡率。结果 三个实验组的仔鼠平均体重分别为 5 .5 46g ,5 .377g ,5 .1 0 3g ,以正常对照组平均体重减 2个标准差为IUGR的标准 ,孕鼠产出的IUGR仔鼠发生率分别为 2 7.8% ,42 .2 % ,6 1 .6 % ,仔鼠围产期死亡率分别为 6 1 .1 % ,1 7.8% ,6 .7%。结论 被动吸烟法及更生霉素腹腔注射法均能成功建立IUGR大鼠模型 ,尤以前者更适用于儿科研究。

Objective To evaluate three models of intrauterine growth retardation (IUGR) in the rat for use in long term metabolism and development. Methods Fifteen pregnant rats were divided into 3 groups, in which IUGR was produced by either 1)uterine artery ligation, 2)abdominal injection of Dectinomycin, or 3) passive smoking. Newborn rats were delivered vaginally, and their body weights, rates of IUGR and perinatal death rates were compared. Results The average body weight of the newborn rats in each group was 5.546g, 5.377g, 5.103g; the IUGR rates were 27.8% , 42.2%, 61.6%; the perinatal death rates were 61.1%, 17.8%, 6.7%, respectively. Conclusion Both Dactinomycin abdominal injection and passive smoking can successfully establish an IUGR rat model, IUGR actived through passive smoking has a higher rate of fetal growth retardation and a lower mortality rate, suggesting that this may be a preferred model.