新疆石河子地区绵羊嗜吞噬细胞无形体的检测和16S rRNA序列分析

Detection of Anaplasma phagocytophilum among sheep in Shihezi, Xinjiang Uyghur Autonomous Region, China and analysis of its 16S rRNA gene sequences

目的调查新疆石河子地区绵羊嗜吞噬细胞无形体感染状况以及分析其病原16S rRNA序列特征。方法在石河子地区采集羊血,提取DNA,巢式PCR扩增16S rRNA基因并与GenBank中相应基因序列进行比对分析。结果在109份羊血样本中,检测出阳性样本37份,阳性率为33.94%,检测出的16S rRNA(524bp)基因序列与部分GenBank中嗜吞噬细胞无形体16S rRNA基因序列同源性达99%。结论新疆石河子地区绵羊中存在嗜吞噬细胞无形体的感染。

Objective To investigate the prevalence of Anaplasma phagocytophilum infection among the sheep in Shihezi, Xinjiang Uyghur Autonomous Region, China and to analyze the 16S rRNA gene sequences of An. phagocytophilum. Methods Blood samples were collected from the sheep in Shihezi; total DNA was extracted from these blood samples; the target 16S rRNA gene fragments of An. phagocytophilum were amplified by nest PCR. The sequences of 16S rRNA gene fragments from positive samples were compared with the corresponding gene sequences deposited in GenBank. Results Of the 109 blood samples, 37 （33.94%） were positive with the target gene fragments. The detected 16S rRNA （524 bp） gene sequences had a sequence homology up to 99% with some 16S rRNA gene sequences of An. phagocytophilurn in GenBank. Conclusion The infection with An. phagocytophilum exists among the sheep in Shihezi, Xinjiang Uyghur Autonomous Region, China.