分泌性中耳炎患者血清和积液中炎性介质PAF含量的微观辨证研究

Study of Microcosmic syndrome differentiation PAF on inflammatory mediator in serum and fluid in patient with Secretory Otitis Media

目的:探讨分泌性中耳炎(SOM)患者血清和积液(MEF)中炎性介质血小板活化因子(PAF)含量与SOM中医辨证分型的关系。方法:收集SOM不同辨证分型并与正常对照组对照,测定SOM各证型MEF和血清中PAF含量。结果:SOM湿热型血清和MEF中PAF含量显著高于风热型、痰瘀型和正常组(P=0.000),而风热型则显著低于湿热型却高于痰瘀型(P=0.000),痰瘀型显著低于风热型和湿热型(P=0.000),但与正常组比较差异无统计学意义(P=1.000);SOM风热型和湿热型MEF中PAF含量均显著高于血清(P=0.000),但痰瘀型MEF与血清中PAF含量比较差异无统计学意义(P=0.505)。结论:SOM患者各证型血清和积液中PAF含量变化特征一致,PAF的高表达是湿热型的特征,而中表达则是风热型的特点,痰瘀型以低表达为主;SOM患者湿热型和风热型MEF中PAF含量将比血清中PAF含量变化更具有微观诊断价值。

Objective： To explore the relationship between SOM TCM syndrome differentiation and the levels of PAF in serum and inflammation mediater.Method： The patients with different syndrome were collected,and were compared with healthy control group,PAF content in MEF and serum in SOM of each group were detected.Result： The PAF content in serum and MEF in damp-heat type group was obviously higher than those in wind-heat type,phlegm and blood stasis type and normal group（P=0.000）.The PAF content in serum and MEF in wind-heat type was obviously lower than that in damp-heat type,but obviously higher than those in phlegm and blood stasis type group（P=0.000）.The PAF content in serum and MEF in phlegm and blood stasis type was obviously lower than wind-heat type and damp-heat type but there was no significant difference between phlegm and blood stasis type group and normal group（P=1.000）.The PAF contents in fluid in wind-heat and damp-heat type were obviously higher than those in serum（P=0.000）,but there was no significant difference between PAF content in fluid and PAF in serum in SOM patients with phlegm and blood stasis type.Conclusion： The change characteristics of PAF contents in serum in each type in SOM patient are consistent with that of the PAF content in fluid.The high expression of PAF in serum and fluid in SOM patient is the characteristics of damp-heat type,medium expression is the characteristics of wind-heat type,and phlegm and blood stasis is mainly to showed low expression.The PAF content in MEF in SOM patients with damp-heat and wind-heat type has more microcosmic diagnosis value than PAF content change in serum.