核桃楸皮粗多糖的急性毒性实验研究

A Study on Acute Toxicity of Crude Polysaccharide Extracted from Juglans Mandshurica Maxim to Mice

目的观察核桃楸皮粗多糖对小鼠的急性毒性。方法将5.4 mg/mL的核桃楸皮粗多糖溶液,分别按照40 mL/kg每天3次灌胃,20 mL/kg每天2次腹腔注射,给药1 d后,连续观察7 d。结果实验期间,小鼠的活动与饮食情况未见异常,未出现死亡。尸检各脏器无异常。小鼠的核桃楸皮粗多糖每天最大给药量:腹腔注射2.16 mg/10 g,灌胃6.48 mg/10 g。结论通过两种给药方法证明,核桃楸皮粗多糖对小鼠的毒性作用较低。

Objective To estimate the acute toxicity of crude polysaccharide extracted from juglans mandshurica maxim to mice. Method Crude polysaccharide extracted from juglans mandshurica maxim （concentration of 5.4 mg/mL） was orally administrated to the mice at a dose of 40 mL/kg, three times per day, and was injected intraperitoneally to the mice at a dose of 20 mL/kg, two times per day. After administrated for 1 days,continuous observated 7 days. Result In the course of the test, the activity and diet of mice were normal, and there were no death of the mice. Autopsy revealed nothing remarkable. The maximum dosage of oral administration and the maximum dosage of intraperitoneal injection were 6.48mg/10g and 2. 16 mg/10g respectively. Conclusion Toxic effect of the rude polysaccharide extracted from juglans mandshurica maxim on the mice in the two administration routes was low.