PGPR菌液处理对新疆加工番茄种子萌发的影响

Effect of PGPR Bacterial Liquid on Germination of Xinjiang Processing Tomato Seed

【目的】通过从阿魏根际中筛选分离获得具有ACC脱氨酶活性的PGPR菌株,研究其对加工番茄种子发芽及种子酶活性的影响。【方法】通过不同时间、不同浓度PGPR菌液对新疆加工番茄里格尔(87-5)浸种处理,研究其对新疆加工番茄里格尔种子萌发特性的影响。【结果】菌液A25处理番茄种子在OD600nm处吸光值0.5、浸种4 h,发芽势最好,发芽势为(60±4.0)%,种子α-淀粉酶活力值是4.454 7 mg/(g.5 min);菌液T129处理番茄种子在OD600nm处吸光值0.25、浸种4 h,发芽势最好,发芽势为(62±2.0)%,种子α-淀粉酶活力值为3.570 mg/(g.5 min)。【结论】经PGPR菌液处理里格尔后,种子淀粉酶活性随着时间和浓度的增加出现峰值后递减。

[ Objective]The project aims to obtain 2 PGPR trains with ACC deaminase activity from the ceratoides lateens and Chinese asafetida rhizosphere in the sandy vegetation of Shihezi area and study their impact on seed germination and enzyme activity of the seed of processing tomato. [ Method ] The effects on seed germination were researched with different times and concentrations of soaking the processing tomatoes Liger 87 -5 in Xinjiang. [ Result] The result indicated that using bacterial liquid A25 in the absorbance value of 0.5 at the OD600 nm 4 h of soaking to process tomato seeds, germination potential is the best. The germination potential is 60%, and the seed ot -amylase activity value is 4. 454 7 rag/（ g · 5min） ; bacterial liquid T129 processing tomato seeds in the absorbance value of 0.25 at the OD600 nm 4 h of soaking, the germination potential is the best and the germination potential is 62%, and seed α - amylase activity value is 3. 570 mg/（ g · 5min）. [ Conclusion] After processing the seeds of Liger 87 - 5 with PGPR bacterial liquid, with the time and concentration increased, amylase activity declined after the peak.