C型肉毒梭菌肉毒毒素的提取与鉴定

Extraction and Identification of Botulinum Toxins

【目的】通过对C型肉毒梭菌肉毒毒素的提取与鉴定,为类毒素和抗毒素的制备及抗原性分析奠定基础。【方法】将分离鉴定得到的C型肉毒梭菌通过扩大培养、产毒培养后将产生的肉毒毒素采用除菌过滤、硫酸铵盐盐析、离心、透析、浓缩的方法从产毒培养基中分离提取出来。再将提取的肉毒毒素通过SDS-PAGE鉴定毒素蛋白的分子量。【结果】分离出来的毒素蛋白重链和轻链分别在98和53 KDa左右,与C型肉毒毒素的理论分子量相符。【结论】提取的肉毒毒素是C型肉毒毒素。

[ Objective] The project aims to lay a foundation for the preparation and antigenicity analysis of the toxoid and antitoxic through extraction and identification of the type C clostridium botulinumn. [ Method ] The separated and identified type C clostridium botulinum underwent expanding training, and after that, the procreant poison produced botulinum toxin through such methods as the aseptic filtration, ammonium sulfate salting separation, centrifugal treatment, dialysis and enrichment processes. Then the extracted toxin went through SDS - PAGE for its toxin protein molecular weight to be identified. [ Result ] From the experiments, the abstracted toxin protein heavy chain was about 98 KDA and light chain about 53 KDA, which was consistent with molecular weight of type C botulinum toxin theory. [ Conclusion] What we have extracted is C -type Clostridium botulinum.