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P2Y受体激动剂2-mesADP诱发脊髓背角小胶质细胞[Ca^(2+)]i升高 被引量：2

P2Y receptor agonist induced the increase of [Ca^(2+)]i in cultured dorsal horn microglial cells
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摘要 目的观察体外培养的背角小胶质细胞P2Y受体激活对其[Ca2+]i的影响。方法培养并纯化脊髓背角小胶质细胞,采用激光共聚焦技术观察P2Y受体激活后小胶质细胞[Ca2+]i的变化。结果 P2Y受体激动剂2-mesADP在10μm即可引起小胶质细胞[Ca2+]i升高,当随着2-mesADP浓度增大,小胶质细胞[Ca2+]i升高更为明显。结论体外培养的大鼠脊髓背角小胶质细胞P2Y受体激活可以促进细胞[Ca2+]i升高。 Objective To investigate the effect of 2 -mesADP （P2Y receptor agonist） on the change of [ Ca2＋ ]i in dorsal horn microglial cells. Methods The expression of ox -42 in cultured dorsal horn mi- croglial cells was observed by immunohistochemical staining. 2 - mesADP - induced Ca2 ＋ mobilization in cultured dorsal horn microglia was measured by laser scanning confocal microscopy. Results Cultured dorsal horn microglial cells expressed high level of ox - 42. Furthermore, 2 - mesADP could induce the increase of the Ca2＋ fluorescent intensity in microglial ceils in a dose - dependent manner. Conclusion P2Y receptor agonists 2 - mesADP can induce the increase of [ Ca2 ＋ ] i in dorsal horn microglial cells.
作者 闵娅兰 程年群 田虹 刘爱东 刘晓红 肖智 曾俊伟
机构地区 遵义医学院生理学教研室暨贵州省麻醉与器官功能保护重点实验室
出处 《遵义医学院学报》 2013年第1期10-12,共3页 Journal of Zunyi Medical University
基金 国家自然科学基金资助项目(NO:31000497) 遵义医学院博士科研启动基金项目(NO:2009)
关键词 脊髓背角 小胶质细胞 P2Y受体 Dorsal horn microglial cell P2Y receptor
分类号 R744.5 [医药卫生—神经病学与精神病学]
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参考文献8

  • 1Tozaki - Saitoh H, Tsuda M, Miyata H, et al. P2Y12 recep- tors in spinal microglia are required for neuropathic pain after peripheral nerve injury [ J ]. J Neurosci, 2008,28 : 4949 - 4956.
  • 2Cattaneo M. The P2 receptors and congenital platelet func- tion defects [ J ]. Semin Thromb Hemost, 2005,31 : 168 - 173.
  • 3Costanzi S, Mamedova L, Gao Z G, et al. Architecture of P2Y nucleotide receptors: structural comparison based on sequence analysis, mutagenesis, and homology modeling [ J]. J Med Chem,2004,47:5393 - 5404.
  • 4Kobayashi K, Yamanaka H, Yanamoto F, et al. Multiple P2Y subtypes in spinal microglia are involved in neuro- pathic pain after peripheral nerve injury [ J 1. Glia, 2012, 60(10) : 1529 - 1539.
  • 5闵娅兰,刘晓红,肖智,刘爱东,田虹,曾俊伟.鞘内注射MRS2395对慢性坐骨神经结扎大鼠脊髓背角钙离子接头蛋白-1表达的影响[J].临床麻醉学杂志,2012,28(11):1112-1115. 被引量：6
  • 6Tatur S, Groulx N, Orlov S N, et al. Ca2 + - dependent ATP release from A549 cells involves synergistic autocrine stimulation by coreleased uridine nucleotides [ J ]. J Physi- ol,2007,584( Pt 2) : 419 - 435.
  • 7Lee S, Chung C Y. Role of VASP phosphorylation for the regulation of microglia chemotaxis via the regulation of fo- cal adhesion formation/maturation [ J ]. Mol Cell Neurosci, 2009,42 : 382 - 390.
  • 8Irino Y, Nakamura Y, Inoue K, et al. Akt activation is in- volved in P2Y12 receptor - mediated chemotaxis of micro- glia[J]. J Neurosci Res ,2008,86 : 1511 - 1519.

二级参考文献11

  • 1Piao ZG,Cho IH,Park CK, et al. Activation of glia and mi- croglial p38 MAPK in medullary dorsal horn contributes to tactile hypersensitivity following trigeminal sensory nerve in- jury. Pain, 2006, 121(3) 219-231.
  • 2Hains BC, Waxman SG. Activated microglia contribute to the maintenance of chronic pain after spinal cord injury. J Neurosci, 2006, 26(16): 4308-4317.
  • 3Schreiber KL, Beitz AJ,Wilcox Gin Activation of spinal microglia in a murine model of peripheral inflammation-induced, long-lasting contralateral allodynia. Neurosci Lett, 2008,440(1): 63-67.
  • 4Kobayashi K, Fukuoka T, Yamanaka H, et al. Neurons and glial cells differentially express P2Y receptor mRNAs in the rat dorsal root ganglion and spinal cord. J Comp Neurol, 2006,498(4) :443-454.
  • 5Kobayashi K, Yamanaka H, Fukuoka T, et al. P2Y12 receptor upregulation in activated microglia is a gateway of p38 signaling and neuropathie pain. J Neurosci, 2008,28 (11) : 2892-2902.
  • 6Tozaki-Saitoh H, Tsuda M,Miyata H,et al. P2Y12 receptors in spinal microglia are required for neuropathie pain after pe- ripheral nerve injury. J Neurosci, 2008,28(19) : 4949-4956.
  • 7Hargreaves K, Dubner R, Brown F, et al. A new and sensitive method for measuring thermal nociception in cutaneous hy- peralgesia. Pain,1988,32(1) :77-88.
  • 8Savi P, Herbert JM. Clopidogrel and ticlopidine: P2Y12 adenosine diphosphate-receptor antagonists for the prevention of athero- thrombosi Semin Thromb Hemost, 2005, 31(2) : 174--183.
  • 9胡玉萍,胡益民,王昱,杨建军,李伟彦.坐骨神经结扎大鼠脊髓背根神经节GDNF和NCAM表达的变化[J].临床麻醉学杂志,2010,26(5):427-429. 被引量：4
  • 10李轶聪,李悦,付宝军,刘芳,李恩有.鞘内注射GABA转运体-1抑制剂NO-711对CCI大鼠脊髓背角pERK表达的影响[J].临床麻醉学杂志,2011,27(5):497-500. 被引量：3

共引文献5

同被引文献18

  • 1Nackley A G, Makriyannis A, Hohmann A G. Selective ac-tivation of cannabinoid CB2 receptors suppresses spinal Fos protein expression and pain behavior in a rat model of inflammation [ J ]. Neuroscience, 2003, 119 ( 3 ) : 747 - 757.
  • 2Whiteside G T, Gottshall S L, Boulet J M,et al. A role for cannabinoid receptors, but not endogenous opioids, in the antinociceptive activity of the CB2 - selective agonist, GW405833 [ J ]. Eur J Pharmacol, 2005,528 ( 1 - 3 ): 65 - 72.
  • 3Ibrahirn M M, Deng H, Zvonok A, et al. Activation of CB2 cannabinoid receptors by AMI241 inhibits experimental ncuropathic pain: pain inhibition by receptors not present in the CNS [ J ]. Proc Natl Acad Sci USA,2003,100 ( 18 ) : 10529 - 10533.
  • 4Romero- Sandoval A, Eisenach J C. Spinal cannabinoid receptor type2 activation reduces hypersensitivity and spi- nal cord glial activation after paw incision[J]. Anesthesi- ology,2007, 106:787 - 794.
  • 5Racz I, Nadal X, Alferink J,et al. Crucial role of CB2 can- nabinoid receptor in the regulation of central immune re- sponses during neuropathic pain [ J ]. J Neurosci, 2008, 28:12125 - 12135.
  • 6Anand U, Otto W R, Anand P. Sensitization of capsaicin and icilin responses in oxaliplatin treated adult rat DRG neurons [J ]. Mol Pain, 2010,24 ( 6 ) : 82.
  • 7Wang W,Cao X,Liu C,et al. Cannabinoid WIN 55,212 -2 inhibits TRPV1 in trigeminal ganglion neurons via PKA and PKC pathways[ J]. Neurol Sci,2012,33( 1 ) :79 -85.
  • 8苗爱亮,王小姗.癫痫的诊断及治疗[J].临床内科杂志,2014,13(10):1053-1055.
  • 9Klaft ZJ, Schulz SB, Maslarova A, et al. Extracellular ATP differentially affects epileptiform activity via puri- nergic P2X7 and adenosine A1 receptors in naive and chronic epileptic rats[J]. Epilepsia,2012,53(11) :1 978- 1 986.
  • 10Choi HK, Ryu HJ, Kim JE,et al. The roles of P2X7 receptor in regional-specific microglial responses in the rat brain following status epilepticus[J]. Neurol Sci, 2012,33 (3) : 515-525.

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遵义医学院学报
2013年 第1期

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