MEBO对家兔内毒素血症血液粘度和TNF-α的影响

EFFECT OF MOIST EXPOSED BURN OINTMENT(MEBO) ON BLOOD VISCOSITY AND TUMOR NECROSIS FACTOR-α(TNF-α) PRODUCTION IN RABBITS WITH ENDOTOXEMIA

目的 :检测 MEBO(湿润烧伤膏 )对家兔内毒素血症血液粘度和 TNF- α的影响。方法 :利用 LBY-N6型血液粘度计测定在 1 1 5s-1和 1 0 s-1切变率下的血液粘度。采用 ELISA双抗体夹心法检测 TNF- α水平。结果 :试验组注射内毒素前后血液粘度相比 P>0 .0 5。对照组注射内毒素前后血液粘度相比 P<0 .0 1。内毒素血症 TNF- α水平试验组与对照组相比 P<0 .0 5。结论 :MEBO可改善内毒素血症血液粘度 ,降低内毒素血症 TNF-α水平。

Objective:To examine the effect of MEBO on blood viscosity and TNF α production in rabbits with endotoxemia.Methods:To determine the blood viscosity and TNF α by Type LBY N6 blood viscometer(with change rates of 115 s -1 and 10 s -1 )and TNF α level by ELISA.Results:There was no difference in blood viscosity between prior and posterior to endotoxin injection in test group( P >0 05),but in control gruop,the difference was very significant ( P <0 01).There was also significant difference in TNF α production in endotxemia between test group and control group( P <0 01).Conclusion:MEBO could improve the blood viscosity and reduce TNF α production in endotoxemia.