不同采收期青蒿黄酮和酚酸含量的动态变化研究

Study on the dynamics trends of total flavonoids and total phenolic acids from Artemisia annua L. in different harvesting time

目的研究不同采收期青蒿中黄酮和酚酸类含量的动态变化规律,确定青蒿最佳采收期。方法通过正交试验设计,确定青蒿黄酮和酚酸类化合物的最佳提取条件,采用UV法,测定不同采收期青蒿中黄酮和酚酸类动态含量变化。结果不同采收期青蒿黄酮和酚酸类含量呈现规律性变化,即营养期>初花期>结实期>盛花期。结论如以黄酮和酚酸类化合物作为青蒿药材质量的指标性成分,则青蒿药材最佳采收期应为营养期,该结果为青蒿药材采收期的确定和质量评价提供一定的科学依据。

Objective To study the dynamics trends of total flavonoids and total phenolic acids from Artemisia annua L. in different harvesting time and decide the best harvesting times. Methods The optimum extraction condition of total flavonoids and total phenolic acids was investigated by orthogonal design, and the dynamics content changes of total flavonoids and total phenolic acids from A rtemisia annua L. were determined. Results The contents of total flavonoids and total phenolic acids from Artemisia annua L. changed regularly in different harvesting time. The contents of total flavonoids and total phenolic acids at vegetative period was higher than that in initial flowering stage. That in initial flowering stage was higher than that in fruits developing stage. In fruits developing stage was higher than in the fullbloom stage. Conclusion If total flavonoids and total phenolic acids were slected as active constituents, the vegetative period may be the best harvesting times for A rtemisia annua L.. The research provides a scientific foundation for the fight collecting-time and the quality evaluation of herbal A rtemisia annua L.