急性髓细胞白血病联合化疗药物敏感性及相互作用的研究

Study on sensitivity and interaction of drugs in combination chemotherapy for acute myeloblastic leukemia

目的 :研究急性髓细胞白血病 (AML)联合化疗药物的体外敏感性及化疗药物间的相互作用。方法 :应用甲基噻唑基四唑法 (MTT法 )检测了柔红霉素、三尖杉酯碱、阿糖胞苷、长春新碱和氢化可的松单药与两种药物联合对 2 2例AML患者白血病细胞的细胞毒性 ,统计分析了药物的相互作用。结果 :三尖杉酯碱 (H)与氢化可的松(HC)的协同率最低 ( 4 5 %) ,阿糖胞苷 (Ara -C)与HC的相加作用发生率最低 ( 2 2 7%) ,H与Ara -C的相加作用发生率最高 ( 5 0 0 %) ,H与长春新碱 (VCR)、HC的拮抗作用发生率最高 ,分别为 5 0 0 %和 5 9 1%。结论 :联合化疗药敏试验更有助于指导临床用药。

Objective: To study the sensitivity in vitro and the interaction of drugs in combination chemotherapy for acute myeloblastic leukemia (AML).Methods: Methyl Thiazolyl Tetrazolium (MTT)method was used to detect the cytotoxic effect of daunorubicin,harringtonine,cytosine arabinoside,vincristine and hydrocortisone on leukemic cells of 22 AML patients with single or two drugs combined.The interaction of drugs was analysed by statistics. Results: The cooperative rate of harringtonine (H) and hydrocortisone(HC)was the lowest(4.5%).The incidence of additive effect of cytosine arabinoside and HC was the lowest (22.7%),but it was the highest (50.0%)for H and Ara-C.The incidence of antagenistic action between Hand vincyistine or HC was 50.0% and 59.1% respectively.Conclusion: The sensitive test of combination chemotherapy drugs is more helpful to guide clinical use of the drugs.