再生障碍性贫血患者T细胞亚群及HLA-DR抗原表达的意义

Expression meaning of T cell subsets and HLA DR in patients with aplastic anemia

目的 :检测再生障碍性贫血患者外周血T淋巴细胞亚群CD4 + 、CD8+ 及HLA DRT抗原的表达 ,探讨再生障碍性贫血发病的免疫机制 ,为再障的临床诊断和治疗提供理论依据。方法 :采用免疫组化斑点印迹技术进行检测 ,并用薄层扫描仪对所有班点印迹信号进行扫描。结果 :再障患者CD4 + 降低 ,CD8+ 升高 ,CD4 + /CD8+ 比值倒置 ,HLA DR表达增高。结论 :T淋巴细胞亚群比例失衡及HLA

Objective: Checking the patients with aplastic anemia expression of T cell subsets CD 4 +,CD 8 +and HLA DR,detect aplastic anemia casued immunol mechanism,in order to clinic diagnosis and therapy of aplastic anemia provited theory basis. Methods: Using immunohistochemical and dot blot to check and using thin layer chromatogrophy scaner to show the strength of dot blot signals. Results: Patients with aplastic anemia CD 4 +level significantly decreased,the level of CD 8 +significantly increased,CD 4 +/CD 8 + reversed,HLA DR expression significantly increased. Conclusion: The proporition imbalance of T cell subsets and expression increased of HLA DR may play a role in the progression of aplastic anemia.