PCR-SSCP对非小细胞肺癌P^(16)基因第二外显子突变的研究

Silver Stain Polymerase Chain Reaction and Single Strand Conformation Polymorphism Analysis Detect Mutations in Exon 2 Tumor Suppressor Gene P^(16) in Human Non small Cell Lung Cancers

目的 检测非小细胞肺癌 P1 6 基因第二外显子突变与临床病理类型和预后的关系。方法 用多聚酶连反应——单链构象多态性 (PCR- SSCP)银染技术。分析 P1 6 / MTS1 基因第二外显子的突变规律。结果 NSCL C肺癌中 P1 6基因第二外显子突变率为 1 7.5 % (7/ 40 ) ,均为 期病例。结论 P1 6基因第二外显子突变可能是 NSCL C发生发展的原因之一 。

Objective To examine P 16 gene mutation in exon 2 in human non small cell lung cancers,and to observe the relationship between P 16 gene mutation and the clinical stage and progression in human non small cell lung cancers.Method Mutation in exon 2 of P 16 gene were screened by polymorphism(PCR SSCP) analysisingwith non isotopie silve staining method in 40 cases of benign lung diseases.Result The mutation rate of P 16 gene in exon 2 in human non small cell lung cancers is 17.5%(7/40).Benign lung disease is zero(0/40),respectively.Significant difference existed in the clinical stage.Mutation in the exon 2 of P 16 gene were found in clinical stage Ⅲ(7/8).Conclusion These results suggest that mutation in exon 2 of P 16 /mts1 gene would be one of tumor genic factors and clinical significance in detecting prognosis in NSCLC. [