自体外周血干细胞移植对乙肝相关终末期肝病患者免疫功能的调节作用

Immunoregulation of autologous peripheral blood stem cell transplantation in patients with hepatitis B-related end-stage liver disease

目的探讨自体外周血干细胞移植对乙型肝炎病毒(HBV)相关终末期肝病患者外周血中T淋巴细胞亚群和细胞因子的免疫调节作用。方法采用流式细胞术检测患者外周血中Th1、Th2、调节性T细胞(Treg)的百分比,ELISA检测血浆IFN-γ、IL-6、IL-10水平。结果自体外周血干细胞移植能明显改善HBV相关终末期肝病患者肝功能,同时上调患者外周血中Th2、Treg的百分比,下调血浆IL-6和IL-10水平(P<0.05),而对Th1细胞及其细胞因子IFN-γ影响无统计学意义。结论自体外周血干细胞移植可能是通过调节机体免疫微环境,抑制肝脏炎症反应,从而改善HBV相关终末期肝病患者肝功能。

Objective To investigate the immunoregulatory effect of autologous peripheral blood stem cells （PBSCs） transplantation on T lymphocytes and cytokines in patients with HBV-related end-stage liver disease. Methods Row cytometry was used to measure the percentages of ThI, Th2 and regulatory T cells （Treg） in peripheral blood. Enzyme-linked immunosor- bent assay （ELISA） was performed to analyze the levels of serum IFN-γ, IL-6 and IL-10. Results Patients with HBV-related end-stage liver disease displayed significantly improved liver function after PBSCs transplantation. Statistically, after PBSCs transplantation, the percentages of Th2 and Treg in peripheral blood markedly increased, but serum IL-6 and IL-10 declined significantly. No significant differences were observed in the changes of Th1 and its cytokine, IFN-γ after transplantation. ConclusionAutologous PBSCs transplantation can depress inflammation in liver by regulating immune microenvironment, which at least in part delineates the mechanism of stem cells-mediated therapeutic benefit on end-stage liver disease.