摘要
目的探讨自体外周血干细胞移植对乙型肝炎病毒(HBV)相关终末期肝病患者外周血中T淋巴细胞亚群和细胞因子的免疫调节作用。方法采用流式细胞术检测患者外周血中Th1、Th2、调节性T细胞(Treg)的百分比,ELISA检测血浆IFN-γ、IL-6、IL-10水平。结果自体外周血干细胞移植能明显改善HBV相关终末期肝病患者肝功能,同时上调患者外周血中Th2、Treg的百分比,下调血浆IL-6和IL-10水平(P<0.05),而对Th1细胞及其细胞因子IFN-γ影响无统计学意义。结论自体外周血干细胞移植可能是通过调节机体免疫微环境,抑制肝脏炎症反应,从而改善HBV相关终末期肝病患者肝功能。
Objective To investigate the immunoregulatory effect of autologous peripheral blood stem cells (PBSCs) transplantation on T lymphocytes and cytokines in patients with HBV-related end-stage liver disease. Methods Row cytometry was used to measure the percentages of ThI, Th2 and regulatory T cells (Treg) in peripheral blood. Enzyme-linked immunosor- bent assay (ELISA) was performed to analyze the levels of serum IFN-γ, IL-6 and IL-10. Results Patients with HBV-related end-stage liver disease displayed significantly improved liver function after PBSCs transplantation. Statistically, after PBSCs transplantation, the percentages of Th2 and Treg in peripheral blood markedly increased, but serum IL-6 and IL-10 declined significantly. No significant differences were observed in the changes of Th1 and its cytokine, IFN-γ after transplantation. ConclusionAutologous PBSCs transplantation can depress inflammation in liver by regulating immune microenvironment, which at least in part delineates the mechanism of stem cells-mediated therapeutic benefit on end-stage liver disease.
出处
《细胞与分子免疫学杂志》
CAS
CSCD
北大核心
2013年第4期407-410,共4页
Chinese Journal of Cellular and Molecular Immunology
基金
国家高技术研究发展计划(863)资助(2011AA020111)