当归对人红细胞变形性和聚集性的影响

Effects of Angelica on Deformability and Aggregation of Human Erythrocytes

目的 :研究当归对红细胞变形性和聚集性影响。方法 :健康献血者红细胞 ,自身血浆稀释至红细胞压积为 40 %。用红细胞聚集仪和激光衍射光学旋转细胞分析仪分别测定红细胞聚集性和变形性。结果 :当归组加入当归注射液 2 0mg/ml孵育后与正常对照组比较 ,可显著降低红细胞聚集速度 (Ta :2 .5 9± 0 .35 ,Tf:33.5 4± 2 .6 3vsTa :1.80±0 .16 ,Tf:2 7.11± 1.78,P <0 .0 5 ) ,但不影响红细胞解聚能力。加入Ca2 + 螯合剂可使红细胞变形性降低。当归可以显著逆转A2 3187导致的红细胞变形性降低 (EI:0 .2 9±0 .0 47vs 0 .17± 0 .0 2 4,P <0 .0 1)。结论 :当归可以降低正常红细胞聚集速度 ,减轻Ca2 + 螯合剂导致的红细胞变形性降低。

Objective: The effects of Angelica on deformability and aggregation of human erythrocytes were studied.Method: The heparinized venous blood from healthy blood donor was diluted to a haematocrit of 40%. The aggregation and deformability of erythrocytes were measured by erythroaggregametre and LORCA.Results: When erythrocytes were incubated for 60 min at 37℃ with and without Angelica, the velocity of aggregation was much slower in group with Angelica than without Angelica(Ta: 2.59 ± 0.35 , Tf: 33.54 ± 2.63 vs Ta: 1.80 ± 0.16 , Tf: 27.11 ± 1.78 , P < 0.05 ). The defrmability was determined by LORCA system. The erythrocytes were incubated with A23187 ( 0.19 μmmol/L) for 60 min at 37℃, the values of EI was smaller than without A23187(EI54: 0.17 ± 0.024 vs 0.55 ± 0.017 , P < 0.001 ). Angelica were found to protect against loss of deformability caused by A23187, the values of EI of the deformability was increased under shear stress (EI54: 0.29 ± 0.047 , P < 0.02 ).Conclusion: These results suggest that Angelica may decrease the aggregation of the normal erythrocytes, reduce the injury of the deformability by Ca 2+ ionophore. It is a beneficial effect for cardiovascular disease and diabetes.