肿瘤坏死因子受体基因单核苷酸多态性与肺炎严重程度的相关性

Relationship between SNP of TNFR gene and incidence/severity of pneumonia

目的:比较肿瘤坏死因子受体(tumor necrosis factor receptor,TNFR)基因多个等位基因在肺炎人群中的分布频率,分析基因多态性与肺炎发病率和病情严重程度的相关性。方法:纳入66例肺炎患者与66例既往无肺炎的健康体检者,抽提各研究对象外周血DNA,通过聚合酶链式反应-限制性片段长度多态性或基因测序的方法检测TNFR1+36A/G、TNFR1-609G/T、TNFR2+676T/G、TNFR2+1663T/G、TNFR2+1668A/G和TNFR2+1690C/T各多态性位点在肺炎患者与健康体检者、重症肺炎与非重症肺炎患者中的分布频率,并统计分析各基因分布频率与肺炎发生率和严重程度的相关性。结果:TNFR1-609G与T等位基因在肺炎患者中分布频率分别为40.9%与59.1%,在健康体检者中的分布频率分别为53.8%与46.2%;TNFR1-609T等位基因在肺炎患者中的分布频率较高(P<0.05)。余基因的各等位基因在肺炎患者与健康体检者中的分布频率差异无统计学意义。TN-FR1-609G与T等位基因在重症肺炎患者中分布频率分别为25.0%与75.0%,在非重症肺炎患者中的分布频率分别为46.0%与54.0%,T等位基因在重症肺炎患者中的分布频率较高(P<0.05)。TNFR2+1690C与T等位基因在重症肺炎患者中分布频率分别为81.1%与18.9%,在非重症肺炎患者中的分布频率分别为61.0%与39.0%,C等位基因在重症肺炎患者中分布频率较高(P<0.05),余基因的各等位基因在重症肺炎与非重症肺炎患者中的分布频率差异无统计学意义。结论:携带TNFR1-609T等位基因的个体更易罹患肺炎,而携带TNFR1-609T及TNFR2+1690C等位基因的个体肺炎病情较严重,易进展为重症肺炎。

AIM： To analyze the relationship between the single nucleotide polymorphism （SNP） of tumor necrosis factor receptor （TNFR） gene and the incidence and severity of pneumonia. METHODS： Total 132 Chinese indi- viduals were enrolled in this study. There were 66 patients with pneumonia and 66 healthy subjects. The SNPs of TNFR gene including TNFR1 ＋ 36A/G, TNFR1- 609G/T, TNFR2 ＋ 676T/G, TNFR2 ＋ 1663T/G, TNFR2 ＋ 1668A/G and TNFR2 ＋ 1690C/T were genotyped by polymerase chain reaction-restriction fragment length polymorphism or gene sequen- cing for all subjects. Polymorphisms affecting pneumonia incidence and severity were calculated by SPSS. RESULTS： The frequencies of TNFR1 -609G and T alleles in pneumonia patients were 40.9% and 59.1%, while those in healthy sub- jects were 53.8% and 46.2%. The frequency of TNFR1 -609T in pneumonia patients was higher than that in healthy sub- jects （P 〈0. 05）. Besides, the frequencies of TNFR1 -609G and T alleles in severe pneumonia patients were 25.0% and 75.0%, while those were 46.0% and 54.0% in non-severe pneumonia patients. The frequencies of TNFR2 ＋ 1690C and T alleles in severe pneumonia patients were 81.1% and 18.9%, while those were 61.0% and 39.0% in non-severe pneu- monia patients. The frequencies of TNFR1 - 609T and TNFR2 ＋ 1690C in severity pneumonia subjects were higher than those in mild subjects （P 〈0. 05）. CONCLUSION： It appears that TNFR1 -609T is associated with high incidence of pneumonia. TNFR1 -609T and TNFR.2 ＋ 1690C are the risk factors of severity in pneumonia in Chinese.