骨桥蛋白在中枢神经系统白血病患者中的表达及意义

Expression of Osteopontin in Central Nervous System Leukemia and Its Clinical Significance

本研究旨在探讨骨桥蛋白(OPN)在中枢神经系统白血病(CNSL)患者中的表达,了解其对CNSL的临床意义。采用ELISA法检测62例儿科患者血清中OPN的表达及19例CNSL患者完全缓解(CR)后OPN的表达,用RT-PCR检测CNSL患者骨髓中OPN的表达变化。结果表明,ELISA检测出OPN在CNSL、急性白血病(非髓外浸润)、非肿瘤患者中水平分别是25.21±6.87、13.24±2.73和3.14±1.60 ng/ml,差异具有显著性;在19例CNSL完全缓解后OPN水平是4.35±1.50 ng/ml,较治疗前明显降低(P<0.001);RT-PCR显示OPN在CNSL患者骨髓中表达较其他2组高(P<0.01)。结论:OPN在白血病中枢神经系统浸润中起着一定的作用,其机制有待临床进一步探讨。

This study was aimed to investigate the expression of osteopontin （OPN） in central nervous system leukemia （CNSL） and to understand its clinical significance. The expression level of OPN in serum of 62 pediatric patients （22 cases of CNSL, 20 cases of acute leukemia without extramedullary infiltration and 20 cases of nontumor patients） and 19 cases of CNSL with complete remission（ CR） were assayed by ELISA; the expression changes of OPN mRNA in bone marrow of the CNSL patients were detected by RT-PCR. The results indicated that the serum OPN level was significantly higher in CNSL group （ 25.21 ±6.87 ng/ml） than that in acute leukemia group（ 13.24±2.73 ng/mi） （P 〈 0.001 ） and nontumorous group （3.14 ± 1.60 ng/ml） （P 〈 0.001 ） ; the serum OPN level （4.35 ±1.50 ng/ml） in CNSL group with CR decreased obviously （P 〈 0. 001 ） after therapy; RT-PCR analysis showed that the expression of OPN mRNA was higher in CNSL group as compared with other two groups （ P 〈 0.01 ）. It is concluded that the OPN expression may play a role in central nervous system infiltration of leukemia, the mechanism of which remains to need further clinical exploration.