不同预处理方法对木薯渣水解效果影响的研究

Effects of pretreatment methods on hydrolysis of cassava residue

为了提高木薯渣发酵效率,采用稀酸、碱、加压热水、酶等13种不同预处理方法分别对木薯渣进行水解。将固相中微生物可利用的有机质转移到液相中,考察了木薯渣不同预处理方法水解液中单糖、低聚糖和总糖浓度的变化。结果表明,木薯渣稀酸水解效果均较好,其中,0.5%HNO3高温水解效果最好,葡萄糖和木糖浓度分别达22 606 mg/L和1 509 mg/L,低聚糖浓度达7 856 mg/L,总糖浓度达33 683 mg/L;碱法和加压热水法水解液中单糖浓度均较低;酶法水解中,半纤维素酶协同纤维素酶法水解效果比单一酶法水解效果好,稀酸强化酶法水解效果较直接进行酶解效果好,其中1%H2SO4强化半纤维素酶协同纤维素酶法水解效果最好,水解液中葡萄糖和木糖浓度分别达19 758 mg/L和1 504 mg/L,低聚糖浓度达19 853 mg/L,总糖浓度达43 196mg/L。

To increase the fermentation efficiency of cassava residue, 13 pretreatment methods were used to hydrolyze cassava residue. By transferring organic matters into the liquid phase from the sol- id phase, the concentration of monosaccharides, oligosaccharides and total sugar in the hydrolysate from different pretreatment methods were investigated. The results showed that all the treatment methods were effective, with the weak acid of 0. 5% HNO3 at high temperature being the best, yield- ing a concentration of glucose and xylose in the hydrolysate of 22 606 mg/L and 1 509 mg/L, oligo- saccharides at 7 856 mg/L, and total sugar at 33 682 mg/L. The effect of hydrolysis for cassava res- idue with NaOH and hot water pressure were not obvious. In enzymatic hydrolysis, cellulase with the addition of emicellulase was more preferable than only single enzymes, and cellulase with the addi- tion of weak acid was more preferable than hydrolysis with enzymes directly, cellulase and emicellu- lase with 1% H2SO4 gave the best results with glucose and xylose in the hydrolysate at 19 757 mg/L and 1 504 mg/L respectively, oligosaccharides at 19 853 mg/L, and total sugar at 43 196 mg/L.