产喹诺里西啶碱苦豆子内生真菌的筛选与鉴定

Screening and identification of quinolizidine alkaloid-forming of endophytic fungi from Sophora alopecuroides

从健康苦豆子植株中分离到214株内生真菌,通过薄层色谱法检测筛选到4株内生真菌产喹诺里西啶碱.用3种培养基分离菌株,根据菌株的菌落形态、大小、颜色、生长速率、质地、生长培养基颜色变化以及菌丝体和孢子的形态特征进行鉴定.结果表明:4株内生真菌分别属于亚大茎点霉属(Macrophoming)、卵形孢霉属(Oospora)、镰孢霉属(Fusarium)和头孢霉属(Cephalosporium).

The 214 endophytic fungi were isolated from healthy Sophora alopecuroides.Four strains of endophytic fungi which produced quinolizidine alkaloid were screened by TLC.The strains were isolated by three kinds of culture mediums.These strains were identified through the colony morphology,size,color,growth rate,texture,color change of growth culture mediums and the morphological characteristics of mycelia and spores.The results showed that they are Macrophoming,Oospora,Fusarium and Cephalosporium.