平菇培养料青、绿霉菌的分离鉴定及特性

Isolation, identification and characteristics of Penicillium and Trichoderna from substrate and contaminated oyster mushroom

从南宁市平菇栽培原料、污染菌包、污染菌种和霉变子实体中分离青、绿霉菌，获得菌株47个，采用形态学比较观察和rDNAITS1—5．8S—ITS2基因片段进行序列分析，结果共鉴定出侧耳木霉、绿色木霉、哈茨木霉、桔青霉、托姆青霉和短密青霉6个种。6个菌株均在40℃以上死亡，湿度在40％以下不能生长，pH值高抑制其生长。

Forty-seven strains of Penicillium and Trichoderma were isolated from materials,substrate bags,spawn and fruiting body of oyster mushroom Pleurotus sp. from Nanning mushroom farms. They were identified to 6 species of Trichoderma pleuroticola, Hypocrea virens, Hypocrea lixii, Penicillium citrnum Thom, Penicillium thomii and PeniciUium brecicompactum according morphological characteristics and rDNA ITS1-5.SS-ITS2 sequences.ln order to control these strains rightly, the text was also focused on the clarificatiom of their corresponding biological characteristics.Their growth optimum temperatur and pH was 25℃ and 6-7,resperctively.They could produce conidia easily at high temperatures and urder dry conditions.