青蒿中黄酮、多酚和V_C含量测定及抗氧化性研究

Determination of Flavonoids,Polyphenol and Vitamin C and Its Antioxidantn Properties of Artemisia annua L.

紫外-可见分光光度法测定了干、鲜青蒿叶中黄酮、多酚和VC的含量,用DPPH.法、水杨酸法和邻苯三酚法对比研究了其抗氧化能力。结果表明,干青蒿叶中黄酮、多酚和VC的含量远高于鲜青蒿;且干青蒿叶提取液抗氧化能力远大于鲜青蒿叶提取液抗氧化能力。当提取液体积为1mL时:DPPH.法清除总自由基能力,干青蒿(92.53%)>鲜青蒿(7.58%);水杨酸法清除羟自由基的能力,干青蒿(31.45%)>鲜青蒿(21.73%);邻苯三酚法清除过氧阴离子的能力,干青蒿(76.36%)>鲜青蒿(18.58%),提取液的抗氧化能力随提取液体积增大而增加,二者呈现一定的量效关系。

The flavonoids ,polyphenol and vitamin C content are determined in the extracts of dry,fresh leaves of arteminsia anntta L. using ultraviolet-visible spectrophotometry and its antioxidant capacity are comparative- ly investigated with DPPH. method,salicylic acid and pyrogallol methods. The results showed that：the flavonoids,polyphenols,and Vc contents in dry leaves of Artemisia annua L was much higher than in fresh Artemisia artnua L; and the dry leaf extract of Artemisia annua L antioxidant capacity was more than fresh leaves extract. Antioxidant capacities with 1 mL of extracts volume of A rtemisia annua L was 92.53 % （dry）〉 7.58 % （fresh）with DPPH method, and 31.45 % （dry）〉21.73 % （fresh）with salicylic acid method, and 76.36 % （dry） 〉18.58 % （fresh）with pyrogallol method,respectively, free radical scavenging capacity of extract of Artemisia annua L was increased with the extract volume increased. There were some quantity-effect relation- ship for .both extracts of Artemisia annua L and scavenging capacity of free radicals.