新型希夫碱铜三元配合物与DNA相互作用的研究

Studies on the Interaction between a Novel Schiff Base Copper(Ⅱ) Triple Complex with DNA

合成了铜(Cu)-噻吩-2-甲醛缩L-蛋氨酸亚胺-1,10-邻菲啰啉(phen)三元配合物,并通过元素分析、红外光谱、紫外-可见光谱、核磁共振及TG-DTG等手段对其结构和化学组成进行表征,确定其化学组成为[CuL(Phen)(H2O)].Ac.2H2O(L:C10H12NO2S2)。利用紫外-可见吸收光谱、荧光光谱、粘度测试研究了配合物与DNA的相互作用,结果表明,配合物与DNA之间通过插入作用结合。

A novel copper （II） triple complex was and thiophene - 2 - formaldehyde - L - methionine imine, synthesized with cupric acetate, 1,10 - phenanthroline （phen） which was characterized by elemental analysis, IR spectrometry, UV- Vis spectrometry, 1H NMR and TG- DTG, and its structure was confirmed to be [ CuL（Phen）（H20）] Ac 2H2O（ L = C10H12NO2S2）. The interaction of the Cu（ 11 ） complex with DNA was investigated by UV -Vis spectrometry, fluorescence spectrometry and viscosity test. The results showed that the combination of the Cu （ II ） complex with DNA was through intercalation mode.