响应面法优化脂肪酶水解葵花油工艺

Optimization of Hydrolysis Sunflower Oil with Lipase by Response Surface Methodology

采用响应面试验设计,在无溶剂体系中,对脂肪酶水解葵花油的生产工艺进行优化,以水解温度、pH、酶添加量为试验因子,以水解率为响应值,建立数学模型。结果表明,最佳水解工艺条件为:水解温度42℃,pH=9.8,酶添加量0.014 mg/g。水解率的模型的预测值为79.53%,实际得到的水解率为80.91%。所得回归模型拟合情况良好,达到设计要求。

Enzymatic hydrolysis of sunflower oil catalyzed by lipase was investigated in a solvent-free system. Using response surface experimental design with hydrolysis temperature, pH, enzyme dosage for the trial factor, the hydrolysis rate was the response value, a mathematical model of enzymatic hydrolysis conditions were optimized. The results showed that the optimal hydrolysis conditions were： hydrolysis temperature 42℃, pH = 9.8, the enzyme dosage 0.01d mg/g. Under optimal conditions, the predicted hydrolysis rate was 79.53 % , the experimental hydrolysis rate was 80.91%. Derived from regression model fitting in good condition and meet the design requirements.