嗜酸乳杆菌与传统混合菌混合培养条件的优化

Optimizing Conditions for Mixed Culture of Lactobacillus Acidophilus and Traditional Yoghourt Strains

研究嗜酸乳杆菌(LA-5)与嗜热链球菌、德氏乳杆菌保加利亚亚种两种传统菌株混合发酵制备益生菌酸乳,正交试验结果得到最适生长条件为:菌种配比为1∶4、接种量0.3 g/L、加糖量60 g/L、培养温度41℃;同时测定所得益生菌酸乳的黏度、酸度、pH以及酸乳中嗜酸乳杆菌活菌数等在发酵以及后酵乃至贮存期间的变化情况。

In this study Lactobacillus aciclophilus （LA-5） is co-cultured with the two traditional yoghourt strains-Streptococcus thermophilus （St） and Lactobacillus bulgaricus （Lb）. Through using the orthogonal test, the optimal fermentation condition is obtained： optimal inoculation mixed proportion of La to St ＆ Lb 1：4; the total adding strain 0.3 g/L, the sugar-adding amount 60 g/L; cultural temperature 41℃. The viscosity, acidity, pH value and LA-5 viable count of final productsas had been judged during fementation, post-fermentation and even storage period.