摘要
目的探讨miR-145在宫颈上皮内瘤变Ⅲ级(CINⅢ级)和宫颈鳞癌患者组织中的表达情况及与宫颈鳞癌发生之间的关系。方法 2010年11月—2011年8月在内蒙古医科大学第一附属医院提取49例宫颈鳞癌组织(宫颈癌组)、27例宫颈上皮内瘤变Ⅲ级和2例原位癌(CINⅢ级组)、11例宫颈良性病变患者(健康对照组)组织中的miRNA,用实时定量PCR的方法分析miR-145在各组患者组织中的表达情况。结果 (1)宫颈鳞癌患者组织中miR-145的表达较健康对照组、CINⅢ级组降低(P<0.05),miRNA-145在宫颈癌组织中的表达是CINⅢ级组的0.579倍,是健康对照组的0.521倍;(2)miR-145在宫颈鳞癌Ⅲ～Ⅳ期与Ⅰ～Ⅱ期以及在宫颈鳞癌高、中分化与低分化组的表达均无明显差异(P>0.05)。结论 miR-145在宫颈鳞癌组织中存在异常表达,提示其与宫颈鳞癌的发生及进展可能有关,值得进一步研究。
Objective To explore the expression of miR-145 in cervical intraepithelial neoplasia Ⅲ level （CIN Ⅲ level） and cervical squamous carcinoma patients tissue and cervical squamous carcinoma, and to explore the relationship between the occurrence. Methods Real time RT-PCR method was used to assess the abundance of miR-145 in 49 cases of the cervical cancer tissues, CINⅢ levels tissues （includes CINⅢ levels 27cases and carcinoma 2 cases） and normal tissues （11 resectable cases）, which collected from Inner Mongolia Medical University Affiliated First Hospital. Results （ 1 ） The expression of the miR-145 was lower in the tissues of cervical cancer compared with that in the CINⅢ levels diseases and normal control （ P 〈 0.05 ）. （2） No significant difference of miR-145 level was observed between different clinical stages （stage Ⅲ and stages Ⅲ -Ⅳ） , different pathological type stage G1, stage G2 and stage G3 （ P 〉 0.05 ）. Conclusion The miR-145 in cervical squamous carcinoma tissue existed abnormal expression, suggested that it＇ s related with cervical squamous carcinoma of the occurrence and progress, deserves further research.
出处
《疑难病杂志》
CAS
2013年第5期359-361,共3页
Chinese Journal of Difficult and Complicated Cases
