中华眼镜蛇毒组分C与传统TACE化疗药体外抑制人肝癌细胞株作用的差异

The Difference of the in vitro Inhibition Effect on Human Hepatocarcinoma Cell Line between Fraction C from Naja Naja Actra Venom and Traditional TACE Chemotherapeutical Drugs

目的 :研究中华眼镜蛇毒组分C(FractionCfromNajaNajaActraVenom ,FC)体外对人肝癌细胞株的细胞毒作用和机理 ,并与传统肝动脉插管栓塞 灌注化疗 (TranscatheterArterialChemoembolization ,TACE)药进行比较。方法 :应用MTT法、流式细胞仪等方法 ,观察FC对人肝癌细胞株 (Bel 74 0 2 )、人支气管上皮细胞株 (HBE16 )及人羊膜细胞株 (HAM )的细胞毒作用、量效关系、凋亡率等 ,并与 5 氟尿嘧啶 (5 Fu)、丝裂霉素 (MMC)、阿霉素 (ADM )等对上述细胞的细胞毒作用进行比较。结果 :FC对人肝癌细胞有明显的抑制作用 ,其 2 4及 4 8h的IC50 分别为 4 .34和 3.96 μg ml,且呈良好的量效关系 ,而正常人支气管上皮细胞 (HBE16 )的IC50 则分别高达10 4 6和 11.82 μg ml,两细胞株间存在差异显著性 (P <0 .0 1)。FC还能诱导肝癌细胞凋亡 ,凋亡率随浓度升高而增加。结论 :在本实验中 ,FC能有效抑制肝癌细胞株的生长 ,抑制作用与浓度呈正相关 ,且能诱导肝癌细胞凋亡 ,与传统TACE化疗药相比较 ,FC是有效的、能选择性抑制肝癌细胞生长的新型抗癌药物。

Objective: To investigate the cytotoxic effect of Fraction C from Naja Naja Actra Venom (FC) on Human Hepatocarcinoma cell line (Bel 7402) and its possible mechanisms and to compare its effect with those of traditional Transcatheter Arterial Chemoembolization (TACE) drugs. Methods: MTT and Flow Cytometry (FCM) were involved in detecting the cytotoxic effects,dose effect related rates and apoptosis rates of FC on Human Hepatocarcinoma cell line(Bel 7402) ,Human Bronchial cell line(HBE 16) and Human Amniotic Membrane cell line(HAM). These effects were compared with those of 5 Fluorouracilum(5 Fu),Mitomycine C(MMC) and Adriamycin(ADM) on the above mentioned cell lines. Result: The inhibitive effect of FC on Bel 7402 was obvious, with the IC 50 of 4.34 and 3.96μg/ml at 24 and 48h respectively, compared to the IC 50 of 10.46 and 11.82 μg/ml respectively for HBE 16. The difference was significant (P<0.01). FC could also induce the apoptosis of Bel 7402,with a good dose effect correlation. Conclusion:FC can effectively inhibit the growth and induce the apoptosis of the Human Hepatocarcinoma cell line (Bel 7402). It suggests that FC is more effective and selective in inhibiting the growth of hepatocarcinoma cells than the traditional TACE chemotherapeutical drugs.