应用柱前衍生化方法测定中生霉素(751)在蔬菜、土壤中残留量

Studies For Residual Of 751 In Vegetables And Soil By Before—Column Derivatization Method

本文研究对象—一中生霉素属氨基糖甙类抗生素,紫外光谱为末端吸收,本文测定方法,采用柱前样品与邻苯二甲醛(O—phthalaldehyde缩号OPA),在适当条件下进行衍生化反应,生成具有荧光的产物,再以反相离子对色谱分离后,用荧光检测器检出,由于分离与检出均采用目前最先进的色谱技术,从而使本文建立的方法灵敏度极高,其最小检出量为6.9×10^(-11)gm,重复性好,相对标准偏差CV值为1.2372%,回收率为97.09%,本方法可广泛应用于其它氨基糖甙类抗生素或含伯胺基因的其它化合物的定性定量研究工作。

A procedure is described that uses high-pressure liquid chromatographyfor residual of 751 in vegetables and soil. The analysis by reverse-phir chromatography before-column derivati-zation with O-phthalaldehyde is used to from fluorescent products fordetection. Chromatographic conditons: Column: Du-pont ZORBAX ODS. Solvent: Methanol/Acetic acid/Hexane sulfonic acid. Flow rate: 1. 2ml/min. Detactor: Fluorescece EX: 340nm EM: 425nm. This method is fairly sensitive and reproducible. The minimin detectable quantity 6.9x10^(-11) gm. Recovery more than 97% and standard deviation was less than 2%. The possible scope of this method for analysis of other aminoglycosideantibiotics.