干细胞移植治疗重症肌无力与CTLA-4、乙酰胆碱受体相关性的实验研究

Study of relationship among AChR, CTLA-4 and myasthenia gravis treated by stem cell transplantation

目的探讨干细胞移植治疗重症肌无力(MG)与细胞毒性T淋巴细胞相关抗原-4(CTLA-4)、乙酰胆碱受体(AChR)之间的相关性。方法 40只Lewis鼠随机分为正常对照组、模型对照组、佐剂对照组和干细胞移植组。除正常对照组外,其余三组大鼠均采用腹腔注射MG患者血清的方法制备MG大鼠模型。以ELISA R-eader检测各组血清AChR Ab滴度,流式细胞术检测各组CTLA-4的表达。结果干细胞移植组AChR Ab滴度和CTLA-4水平显著低于模型对照组及佐剂对照组(P<0.01)。结论干细胞移植可调节机体的免疫系统,从而降低血清AChR Ab水平,抑制CTLA-4的表达,为治疗MG提供了重要的理论依据。

【Objective】To investigate the relationship among AChR,CTLA-4 and myasthenia gravis treated by stem cell transplantation.【Methods】40 Lewis rats were divided into control group,model group,adjuvant group and stem cell transplantation group.Serum AChR Ab titre was tested by ELISA Reader,and CTLA-4 by flow cytometry.【Results】AChR Ab titre and CTLA-4 of transplantation group were significantly lower than that of model group and adjuvant group（P 0.01）.【Conclusion】Stem cell transplantation can adjust immune system of organism.So it can reduce the level of AChR Ab and inhibit the expression of CTLA-4.It offers an important theoretical foundation of therapeutic for MG.