大豆种子活力及其与LOX活性相关性的研究

Study on Seed Vigor of Soybean and the Correlation Between Seed Vigor and Activity of Soybean Lipoxidases

选用138个大豆品种(系)为试验材料,采用幼苗生长测定、电导率测定和田间出苗率测定这3种方法测定种子活力,采用分光光度法测定种子LOX活性,并进行各指标间相关分析。结果表明:大豆品种(系)间种子活力及LOX活性存在明显差异,幼苗生长测定的发芽势、胚根长度、鲜重、干重以及简化活力指数均与田间出苗率呈显著或极显著正相关,浸泡液电导率与田间出苗率呈极显著负相关,LOX活性与发芽势、发芽率、胚根长度及绝对电导率均呈极显著正相关,但LOX活性与田间出苗率及其他指标均无相关性。结果表明,幼苗生长测定和电导率测定均可作为大豆种子活力测定的适宜方法,初步认为大豆种子活力与LOX活性间的相关性无明显规律可循。

138 soybean varieties were used as materials and the methods of seedling growth test,electrical conductivity test,field germination test were adopted to test the seed vigor, speetro photowetry was used to measure LOX activity of soybean, and then to analyse the correlation of each index of soybean. The results showed, there were obvious differences of seed vigor and LOX activity between different soybean varieties. Germination energy , radicle length, fresh weight, seedling growth test had a significant or extremely dry weight and simplified vigor index which tested by significant positive correlation with seedling rates in field condition. Electrical conductivity had a significant negative correlation with seedling rates in field condition. LOX activity had a significant positive correlation with germination energy, germination percentage, radicle length and absolute conductivity, but there were no correlation between LOX activity and the other indexes such as seedling rates in field condition. The study identified that seedling growth test and electrical conductivity test were both the suitable methods there was no obvious rule to follow of correlation to test seed vigor of soybean and we preliminary think that between LOX activity and seed vizor of sovbean.